Flow-Injection Chemiluminescence Method for Sensitive Determination of Ascorbic Acid in Fruit Juices and Pharmaceutical Samples Using a Luminol-Cetyltrimethylammonium Chloride Reversed Micelle System

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Tamer H A Hasanin, Terufumi Fujiwara

Abstract

A highly sensitive flow-injection (FI) method was developed for the determination of ascorbic acid using chemiluminescence (CL) based detection. This method involved the following processes: (1) reduction of tetrachloroaurate(III) in hydrochloric acid with ascorbic acid; (2) on-line extraction of the residual Au(III) with rhodamine B from the aqueous hydrochloric acid solution into toluene, followed by the separation of the Au(III)-containing organic phase from the aqueous phase through a microporous Teflon membrane in the flow system; and (3) the measurement of CL produced in a flow cell upon mixing of the extract stream of Au(III) in toluene with luminol in the reversed micellar medium of cetyltrimethylammonium chloride-water in 1-hexanol-cyclohexane, which was injected into a CL reagent stream. In this procedure, a reduction in the CL intensity occurred due to the addition of ascorbic acid to the Au(III) solution. The CL signal of Au(III) decreased with increasing concentration of ascorbic acid in the aqueous sample solution. The proposed procedure allowed the indirect quantitative determination of ascorbic acid in the range of 1.0 × 10-12 to 1.0 × 10-7 M with a correlation coefficient of 0.987 and relative standard deviatio...Continue Reading

References

May 26, 2005·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Takeshi KatoShukuro Igarashi
Jan 25, 2006·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Terufumi FujiwaraTakahiro Kumamaru
Aug 29, 2007·Analytica Chimica Acta·Tatyana N ShekhovtsovaAnna Z Galimova
Oct 31, 2008·Talanta·M C Yebra-Biurrun
Oct 3, 2012·Free Radical Biology & Medicine·Yuta MatsuokaKen-ichi Yamada
Jan 11, 2013·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Tomoyuki YamamotoTerufumi Fujiwara
Oct 20, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Samira Sharifian, Alireza Nezamzadeh-Ejhieh
Feb 11, 2016·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Tamer H A HasaninTerufumi Fujiwara

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Citations

Jan 22, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hongli YangAiping Fan
Jan 22, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Elham MokhtarzadehJavad Hassanzadeh
Apr 16, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Muhammad Shoaib KhanMohammed Yaqoob
Nov 12, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Ryota SatoTakashi Masadome
Feb 11, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Shihua YuYan Gao
Mar 3, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Ze WangQiang Fei

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