Flow-injection chemiluminescent immunoassay for alpha-fetoprotein based on epoxysilane modified glass microbeads

Journal of Immunological Methods
Zhifeng FuHuangxian Ju

Abstract

A flow-injection chemiluminescent immunoassay system based on a novel transparent immunoaffinity reactor is proposed for the quantitation of alpha-fetoprotein. The reactor prepared with alpha-fetoprotein immobilized epoxysilane modified glass microbeads was used as an immunosensor for chemiluminescent detection. With a non-competitive immunoassay format, the proposed immunosensor system is a low cost, flexible and rapid assay for alpha-fetoprotein. After an off-line incubation of the analyte alpha-fetoprotein with horseradish peroxidase-labeled alpha-fetoprotein antibody as enzyme tracer, the mixture was injected into the reactor, which led to trapping of the free enzyme tracer by the reactor. The trapped enzyme tracer was detected by the p-iodophenol-luminol-H2O2 chemiluminescence system. Under optimal conditions, the decrease in chemiluminescence intensity was proportional to the alpha-fetoprotein concentration in the range of 5.0-100 ng/ml with a detection limit of 2.7 ng/ml at a signal/noise ratio of 3. The immunosensor system showed an acceptable reproducibility and stability. Clinical serum samples were assayed with this method and the results were in acceptable agreement with those obtained from immunoradiometric assay.

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Citations

Nov 15, 2008·Chemical Communications : Chem Comm·Ruizhuo OuyangHuangxian Ju
Jun 18, 2009·Chemical Communications : Chem Comm·Liyuan ChenSongqin Liu
Jul 25, 2015·Scandinavian Journal of Clinical and Laboratory Investigation·Fan ZhouTong Wang
Dec 1, 2012·Journal of Healthcare Engineering·Alicia D Powers, Sean P Palecek
Apr 12, 2008·Clinica Chimica Acta; International Journal of Clinical Chemistry·Xu WangJin-Ming Lin
Mar 18, 2016·Biosensors & Bioelectronics·Na Rae JoYong-Beom Shin
Jul 18, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Li LiHe-Ye Wang
May 29, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yan-Ming LiuJie-Ke Cheng
Jan 27, 2009·Biosensors & Bioelectronics·Caifeng DingShusheng Zhang
May 15, 2013·International Journal of Molecular Sciences·Min-Ah WooHyun Gyu Park
Nov 18, 2014·World Journal of Gastroenterology : WJG·Carolina V UlianaHideko Yamanaka
Mar 12, 2011·Electrophoresis·Christian KellnerCiara K O'Sullivan
Oct 6, 2020·Biomedical Optics Express·Haishun LiuMeiyan Liang
Aug 19, 2020·Sensors·Mohamed SharafeldinJames F Rusling
Jan 15, 2009·Analytical Chemistry·Yafeng WuSongqin Liu

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