PMID: 9177077Apr 1, 1997Paper

Flow injection spectrophotometric determination of L-Dopa and carbidopa in pharmaceutical formulations using a crude extract of sweet potato root [Ipomoea batatas (L.) Lam.] as enzymatic source

The Analyst
O Fatibello-Filho, I da Cruz Vieira

Abstract

A flow injection (FI) spectrophotometric method is proposed for the determination of L-dopa and carbidopa in pharmaceutical formulations. After selection of the extraction medium (e.g., buffer-to-tissue ratio, pH, buffer concentration, protective agents and/or stabilizers) and storage conditions, crude extract of sweet potato root [Ipomoea batatas (L.) Lam.] was used as an enzymatic source of polyphenol oxidase (Tyrosinase; catechol oxidase; EC.1.14.18.1) directly in the carrier. This enzyme catalyses the oxidation of these catecholamines to the corresponding dopaquinone. Further, dopaquinone undergoes a rapid spontaneous auto-oxidation to leucodopachrome, which is in turn oxidized to dopachrome; this last compound has a strong absorption at 480 and 360 nm for L-dopa and carbidopa, respectively. For the optimum extraction conditions found the enzyme activity of the crude extract did not vary for at least 5 months when stored at 4 degrees C and decreased by only 4-5% during an 8 h working period at 25 degrees C. The results obtained for L-dopa and carbidopa by the proposed enzymatic FI method were in close agreement with the label values (r1 = 0.9699 and r2 = 0.9999) and also with those obtained using a pharmacopeial method (r3 ...Continue Reading

Citations

May 18, 2005·Journal of Pharmaceutical and Biomedical Analysis·Márcio F BergaminiMaria Valnice B Zanoni
Jun 9, 2001·International Journal of Pharmaceutics·M F Teixeira, O Fatibello-Filho
May 30, 2001·Journal of Pharmaceutical and Biomedical Analysis·L H Marcolino-JúniorO Fatibello-Filho
Jun 3, 2014·The Analyst·Alex D BatistaElias A G Zagatto
May 1, 2004·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Junwei Di, Shuping Bi
Sep 13, 2006·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Jamshid L ManzooriMaghsood Orooji
Jun 21, 2003·Organic Letters·Pieter C DorresteinTadhg P Begley
May 22, 2009·Bioorganic & Medicinal Chemistry Letters·Beata Gasowska-BajgerHubert Wojtasek
Apr 4, 2007·Journal of Pharmaceutical and Biomedical Analysis·Afsaneh Safavi, Maryam Tohidi
Jan 9, 2014·Luminescence : the Journal of Biological and Chemical Luminescence·Prashant P Raut, Shrikant Y Charde
Nov 1, 2005·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Joanna KarpińskaElzbieta Wołyniec
Mar 2, 2010·Pharmaceuticals·Yoshihiro Uesawa, Kiminori Mohri
Jan 7, 2021·Natural Product Reports·Markus GresslerDirk Hoffmeister
Nov 17, 2020·Analytica Chimica Acta·Paulo A Raymundo-PereiraOrlando Fatibello-Filho
Jun 29, 1999·Analytical Chemistry·R K Gilpin, L A Pachla

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