Flow rates in perfusion bioreactors to maximise mineralisation in bone tissue engineering in vitro

Journal of Biomechanics
Feihu ZhaoSandra Hofmann

Abstract

In bone tissue engineering experiments, fluid-induced shear stress is able to stimulate cells to produce mineralised extracellular matrix (ECM). The application of shear stress on seeded cells can for example be achieved through bioreactors that perfuse medium through porous scaffolds. The generated mechanical environment (i.e. wall shear stress: WSS) within the scaffolds is complex due to the complexity of scaffold geometry. This complexity has so far prevented setting an optimal loading (i.e. flow rate) of the bioreactor to achieve an optimal distribution of WSS for stimulating cells to produce mineralised ECM. In this study, we demonstrate an approach combining computational fluid dynamics (CFD) and mechano-regulation theory to optimise flow rates of a perfusion bioreactor and various scaffold geometries (i.e. pore shape, porosity and pore diameter) in order to maximise shear stress induced mineralisation. The optimal flow rates, under which the highest fraction of scaffold surface area is subjected to a wall shear stress that induces mineralisation, are mainly dependent on the scaffold geometries. Nevertheless, the variation range of such optimal flow rates are within 0.5-5 mL/min (or in terms of fluid velocity: 0.166-1.66 ...Continue Reading

Citations

Apr 24, 2020·International Journal for Numerical Methods in Biomedical Engineering·Feihu ZhaoSandra Hofmann
Dec 22, 2019·Applied Biochemistry and Biotechnology·Xueyan HuKedong Song
Jun 14, 2019·Stem Cells International·Alice PasiniEmanuele Giordano
Jan 7, 2021·Tissue Engineering. Part C, Methods·Tommaso RistoriCecilia M Sahlgren
Nov 2, 2019·Acta Biomaterialia·Y LiA A Zadpoor
May 1, 2021·International Journal of Molecular Sciences·Jianhua ZhangRalph Müller
May 7, 2021·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Jasmina StojkovskaDjordje N Veljovic
Jul 9, 2021·Journal of Materials Science. Materials in Medicine·Mehdi MoradkhaniBahram Ahmadian
Sep 2, 2021·Acta Biomaterialia·Yinchuan WangGuangyin Yuan

❮ Previous
Next ❯

Related Concepts

Related Feeds

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.