PMID: 8587857Oct 1, 1995Paper

Flow sorting of fetal erythroblasts using intracytoplasmic anti-fetal haemoglobin: preliminary observations on maternal samples

Prenatal Diagnosis
Y L ZhengD W Bianchi

Abstract

Monoclonal antibody to fetal haemoglobin (alpha 2 gamma 2) has been proposed as a fetal-specific reagent. We developed an intracellular staining protocol that combines fluorescein isothiocyanate or phycoerythrin conjugated anti-gamma with the DNA binding dye Hoechst 33342 to identify and flow sort fetal erythroblasts from maternal blood. Our preliminary observations on anti-gamma-positive cells sorted from four different pregnant women are described here, using fluorescence in situ hybridization (FISH) with chromosome-specific probes to identify fetal cells. Our data demonstrate that far fewer candidate fetal cells are sorted with this protocol than by current cell surface staining methods that employ the monoclonal antibody CD71. This results in increased fetal cell sorting purities. With this protocol, standard FISH techniques require modification due to the rigorous fixation with 4 per cent paraformaldehyde. Our initial data indicate the promise of this approach.

References

May 1, 1992·American Journal of Obstetrics and Gynecology·D Gänshirt-AhlertW Holzgreve
Dec 1, 1991·American Journal of Obstetrics and Gynecology·J O PriceD Shook
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D W BianchiS A Latt
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Citations

Mar 10, 2001·Prenatal Diagnosis·V Martel-PetitP Jonveaux
Jul 31, 1999·European Journal of Obstetrics, Gynecology, and Reproductive Biology·S HahnW Holzgreve
Jun 3, 1999·British Journal of Haematology·D W Bianchi
Jan 28, 2014·PloS One·Taysa Ribeiro SchalcherMarta Chagas Monteiro
Aug 17, 2001·Clinical Chemistry and Laboratory Medicine : CCLM·F Torricelli, C Pescucci
Feb 28, 2003·Pharmacogenomics·Rossa W K Chiu, Y M Dennis Lo
Sep 24, 2010·Clinical & Developmental Immunology·Yi Luo, Matthew J Knudson
Mar 28, 2006·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·C E Van der SchootM de Haas
Sep 14, 2000·Baillière's Best Practice & Research. Clinical Obstetrics & Gynaecology·W Holzgreve, S Hahn
Jan 10, 2003·The Journal of Pediatrics·Bharath SrivatsaDiana W Bianchi
Sep 5, 2013·Biomacromolecules·Sigrid Seuss, Aldo R Boccaccini
Oct 15, 2003·Prenatal Diagnosis·Laird Jackson
Jul 1, 1998·British Journal of Obstetrics and Gynaecology·W L MartinD T Liu
Feb 7, 2007·Microscopy Research and Technique·Kirby L JohnsonDiana W Bianchi
Oct 17, 1998·Journal of Perinatal Medicine·J Gardosi
May 12, 2021·Prenatal Diagnosis·Liesbeth VossaertIgnatia B Van den Veyver

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