Flow-through immunomagnetic separation system for waterborne pathogen isolation and detection: application to Giardia and Cryptosporidium cell isolation

Analytica Chimica Acta
Qasem RamadanHan Hua Feng

Abstract

Simultaneous sample washing and concentration of two waterborne pathogen samples were demonstrated using a rotational magnetic system under continuous flow conditions. The rotation of periodically arranged small permanent magnets close to a fluidic channel carrying magnetic particle suspension allows the trapping and release of particles along the fluidic channel in a periodic manner. Each trapping and release event resembles one washing cycle. The performance of the magnetic separation system (MSS) was evaluated in order to test its functionality to isolate magnetic-labelled protozoan cells from filtered, concentrated tap water, secondary effluent water, and purified water. Experimental protocols described in US Environmental Protection Agency method 1623 which rely on the use of a magnetic particle concentrator, were applied to test and compare our continuous flow cell separation system to the standard magnetic bead-based isolation instruments. The recovery efficiencies for Giardia cysts using the magnetic tube holder and our magnetic separation system were 90.5% and 90.1%, respectively, from a tap water matrix and about 31% and 18.5%, respectively, from a spiked secondary effluent matrix. The recovery efficiencies for Crypto...Continue Reading

Citations

May 15, 2012·Analytical and Bioanalytical Chemistry·Ivo SafarikMirka Safarikova
Mar 26, 2015·Journal of Microbiological Methods·Sofia AnderssonRomanico B G Arrighi
Mar 19, 2014·Water Research·Helen BridleMarc P Y Desmulliez
Apr 14, 2016·Journal of Microbiological Methods·M Jimenez, H Bridle
Jun 27, 2017·Water Research·Artemis EfstratiouPanagiotis Karanis
Oct 5, 2018·Lab on a Chip·Romuald Lejard-MalkiVincent Senez
Oct 27, 2017·Current Medicinal Chemistry·Agnieszka ChylewskaMariusz Makowski

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anxiety Disorders

Discover the latest research on anxiety disorders including agoraphobia, panic disorder, obsessive-compulsive disorder, and post-traumatic stress disorder here.