Flow-through polymerase chain reactions in chip thermocyclers

Journal of Biotechnology
I Schneegass, J M Kohler

Abstract

The miniaturization of analytical devices by micromachining technology is destined to have a major impact on medical and bioanalytical fields. To meet the current demands for rapid DNA amplification, various instruments and innovative technologies have been introduced by several groups in recent years. The development of the devices was extended in different directions and adapted to corresponding applications. In this review the development of a variety of devices and components for performing DNA amplification as well as the comparison of batch-process thermocyclers with reaction chambers and flow-through devices for different purposes are discussed. The main attention is turned to a flow device concept for thermocycling using microfabricated elements for local heat flow management, for which simulations and considerations for further improvement regarding design, material choice and applied technology were performed. The present review article mainly discusses and compares thermocycling devices for rapid thermocycling made of silicon or of silicon and glass with a short excursion to the possibility of plastic chip devices. In order to perform polymerase chain reactions (PCRs) in the microreactors, special attention must be p...Continue Reading

References

Jan 15, 1996·Nucleic Acids Research·M A ShoffnerP Wilding
Oct 15, 1995·Analytical Chemistry·A T Woolley, R A Mathies
Feb 12, 1998·Electrophoresis·C L ColyerD J Harrison
Mar 10, 1998·Analytical Chemistry·A T WoolleyR A Mathies
Mar 21, 1998·Current Opinion in Biotechnology·G R Taylor, P Robinson
Jun 6, 1998·Science·M U KoppA Manz
Oct 17, 1998·Science·M A BurnsD T Burke
Jan 6, 1999·Proceedings of the National Academy of Sciences of the United States of America·H P ChouS Quake
Jan 16, 1999·Current Opinion in Biotechnology·D WhitcombeS Little
Jul 17, 1999·Trends in Biotechnology·D J Ehrlich, P Matsudaira
Jan 14, 2000·Electrophoresis·J C McDonaldG M Whitesides
Jan 15, 2000·Science·R Sikorski, R Peters
Nov 25, 2000·Science·S R Quake, A Scherer
Feb 28, 2001·Fresenius' Journal of Analytical Chemistry·S C JakewayE L Russell
Apr 6, 2001·Electrophoresis·Y Chen, A Pépin
Apr 6, 2001·Electrophoresis·J P AlarieJ M Ramsey
Dec 1, 1998·Analytical Chemistry·D C DuffyG M Whitesides

❮ Previous
Next ❯

Citations

Dec 21, 2006·Biomedical Microdevices·Asensio GonzalezMark Davies
Mar 5, 2009·Biomedical Microdevices·David J KinahanMark R D Davies
Oct 16, 2003·Biosensors & Bioelectronics·Nathaniel C CadyCarl A Batt
Apr 12, 2003·Archives of Histology and Cytology·Yasutaka MatsubaraEiichi Tamiiya
May 17, 2007·Biomedical Microdevices·Olivier FreyJan Lichtenberg
May 17, 2007·Biomedical Microdevices·Tetsuo OhashiYoshiyuki Togawa
Aug 2, 2005·Biotechnology Advances·Pawel L UrbanNeil C Bruce
Feb 1, 2006·Journal of Microelectromechanical Systems : a Joint IEEE and ASME Publication on Microstructures, Microactuators, Microsensors, and Microsystems·Shifeng LiShaochen Chen
Apr 1, 2015·PloS One·Michael S BartschKamlesh D Patel
May 24, 2006·Biomedical Microdevices·Yi-Hsien ChangJr-Lung Lin
Aug 17, 2012·Science Progress·Holger Becker, Claudia Gärtner
Nov 13, 2010·Small·Leslie Y YeoJames R Friend
Jul 15, 2005·Expert Review of Molecular Diagnostics·Götz MünchowKlaus-Stefan Drese
Jan 13, 2006·Biomedical Microdevices·E J WalshA Gonzalez
Feb 29, 2020·Micromachines·Henk-Willem VeltkampJoost Lötters
Mar 19, 2011·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yusuke FuchiwakiHidenori Nagai

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.