Flow Virometry Quantification of Host Proteins on the Surface of HIV-1 Pseudovirus Particles.

Viruses
Jonathan BurnieChristina Guzzo

Abstract

The HIV-1 glycoprotein spike (gp120) is typically the first viral antigen that cells encounter before initiating immune responses, and is often the sole target in vaccine designs. Thus, characterizing the presence of cellular antigens on the surfaces of HIV particles may help identify new antiviral targets or impact targeting of gp120. Despite the importance of characterizing proteins on the virion surface, current techniques available for this purpose do not support high-throughput analysis of viruses, and typically only offer a semi-quantitative assessment of virus-associated proteins. Traditional bulk techniques often assess averages of viral preparations, which may mask subtle but important differences in viral subsets. On the other hand, microscopy techniques, which provide detail on individual virions, are difficult to use in a high-throughput manner and have low levels of sensitivity for antigen detection. Flow cytometry is a technique that traditionally has been used for rapid, high-sensitivity characterization of single cells, with limited use in detecting viruses, since the small size of viral particles hinders their detection. Herein, we report the detection and surface antigen characterization of HIV-1 pseudovirus p...Continue Reading

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Methods Mentioned

BETA
transmission electron microscopy
flow cytometry
light scattering
transfections
ELISA
FCS
sizing
flow virometry
transfection

Software Mentioned

FACS Diva
MIFlowCyt
EV
Gen 5
FCM PASS
CytExpert
FlowJo

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