Flower color modulations of Torenia hybrida by downregulation of chalcone synthase genes with RNA interference

Journal of Biotechnology
Ei-ichiro FukusakiAkio Kobayashi

Abstract

Suppression of biosynthetic genes involved in flower color formation is an important approach for obtaining target flower colors. Here we report that flower color of the garden plant Torenia hybrida was successfully modulated by RNA interference (RNAi) against a gene of chalcone synthase (CHS), a key enzyme for anthocyanin and flavonoid biosynthesis. By using each of the coding region and the 3'-untranslated region of the CHS mRNA as an RNAi target, exhaustive and gene-specific gene silencing were successfully induced, and the original blue flower color was modulated to white and pale colors, respectively. Our results indicate that RNAi is quite useful for modulations of flower colors of commercially important garden plants.

References

Apr 1, 1992·Bio/technology·R HiguchiR Griffith
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M Church, W Gilbert
Dec 29, 2000·Phytochemistry·J B Harborne, C A Williams
Apr 5, 2001·Current Opinion in Biotechnology·G Forkmann, S Martens
Apr 26, 2003·Trends in Biochemical Sciences·Ahmet M Denli, Gregory J Hannon

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Citations

Apr 20, 2011·Die Naturwissenschaften·Umesh Balkrishna JagtapVishwas Anant Bapat
Jul 20, 2007·Plant Cell Reports·Takashi NakatsukaMasahiro Nishihara
Nov 6, 2010·Biotechnology Letters·Masahiro Nishihara, Takashi Nakatsuka
Jan 10, 2014·Planta·Satyajit SaurabhDinesh Prasad
Apr 4, 2014·BMC Plant Biology·Masahiro NishiharaTakashi Nakatsuka
Feb 24, 2005·Bioscience, Biotechnology, and Biochemistry·Chung-Il AnAkio Kobayashi
Apr 15, 2008·Current Opinion in Biotechnology·Yoshikazu Tanaka, Akemi Ohmiya
Apr 20, 2017·International Journal of Molecular Sciences·Lingxia WangWei Chen
Aug 27, 2005·Plant & Cell Physiology·Lucia Cardenas PawloskiRichard B Meagher
Jul 17, 2020·Frontiers in Plant Science·Protiva Rani Das, Sherif M Sherif
Dec 7, 2018·BMC Plant Biology·Masahiro NishiharaKeisuke Tasaki
Aug 28, 2021·International Journal of Molecular Sciences·Emil KhusnutdinovElena Mikhaylova

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