FLS2 is a CDK-like kinase that directly binds IFT70 and is required for proper ciliary disassembly in Chlamydomonas

PLoS Genetics
Qin ZhaoJunmin Pan

Abstract

Intraflagellar transport (IFT) is required for ciliary assembly and maintenance. While disruption of IFT may trigger ciliary disassembly, we show here that IFT mediated transport of a CDK-like kinase ensures proper ciliary disassembly. Mutations in flagellar shortening 2 (FLS2), encoding a CDK-like kinase, lead to retardation of cilia resorption and delay of cell cycle progression. Stimulation for ciliary disassembly induces gradual dephosphorylation of FLS2 accompanied with gradual inactivation. Loss of FLS2 or its kinase activity induces early onset of kinesin13 phosphorylation in cilia. FLS2 is predominantly localized in the cell body, however, it is transported to cilia upon induction of ciliary disassembly. FLS2 directly interacts with IFT70 and loss of this interaction inhibits its ciliary transport, leading to dysregulation of kinesin13 phosphorylation and retardation of ciliary disassembly. Thus, this work demonstrates that IFT plays active roles in controlling proper ciliary disassembly by transporting a protein kinase to cilia to regulate a microtubule depolymerizer.

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Citations

Apr 16, 2021·Current Biology : CB·Kwangjin ParkMichel R Leroux
Apr 30, 2021·Frontiers in Cell and Developmental Biology·Elena A MayDavid U Mick
Nov 12, 2021·ACS Chemical Biology·Zhijun DongJunmin Pan

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Methods Mentioned

BETA
immunoprecipitation
two-hybrid
pull-down
Co-immunoprecipitation
transgenic
PCR
electrophoresis

Software Mentioned

Adobe Photoshop
ZEN
RESDA
Adobe Illustrator
GraphPad Prism
ImageJ

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