Flubendazole interferes with a wide spectrum of cell homeostatic mechanisms in Echinococcus granulosus protoscoleces

Parasitology International
Andrea C CuminoG Denegri

Abstract

The problem of chemotherapeutic treatments for human echinococcosis has not been completely solved. The benzimidazole-methylcarbamates (BZD), broad-spectrum antihelminthic agents, such as mebendazole and albendazole are the only drugs licensed for treatment of hydatid cysts. These drugs bind directly to beta-tubulin causing the disruption of microtubule-based processes in helminths. However, the molecular bases of their multiple biological activities are poorly understood. Recently, the effect of halogenated derivative flubendazole (FLBZ), against E. granulosus larvae has been conclusively demonstrated. The comparative effectiveness of FLBZ, among other BZDs, was shown by means of vitality tests and time of appearance of morphological damage of larvae. In the present study, we examined biochemical and molecular changes on protoscoleces treated with FLBZ. We show that FLBZ induces: 1) an increase in cytosolic free calcium, 2) a decrease in tubulin transcripts, 3) a reduction of mMDH expression and 4) a significant decrease in glycogen levels. These results are consistent with the existence of multiple targets for FLBZ, such as calcium signaling and energy metabolism, and contribute to the understanding of the pharmaceutical prop...Continue Reading

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Citations

Oct 15, 2013·Parasitology International·María Celeste NicolaoAndrea C Cumino
Oct 12, 2013·Veterinary Parasitology·R S Petrigh, M H Fugassa
Sep 6, 2014·Environmental Science and Pollution Research International·Marta WagilStefan Stolte
Apr 12, 2017·Acta Medica (Hradec Králové)·Kristýna ČáňováEmil Rudolf
Oct 13, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Li-Ping DuanHao-Bing Zhang
Jul 31, 2020·International Journal of Molecular Sciences·Barbora VitovcovaEmil Rudolf

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