Fluctuation Analysis of Centrosomes Reveals a Cortical Function of Kinesin-1

Biophysical Journal
Franziska WinklerJörg Grosshans

Abstract

The actin and microtubule networks form the dynamic cytoskeleton. Network dynamics is driven by molecular motors applying force onto the networks and the interactions between the networks. Here we assay the dynamics of centrosomes in the scale of seconds as a proxy for the movement of microtubule asters. With this assay we want to detect the role of specific motors and of network interaction. During interphase of syncytial embryos of Drosophila, cortical actin and the microtubule network depend on each other. Centrosomes induce cortical actin to form caps, whereas F-actin anchors microtubules to the cortex. In addition, lateral interactions between microtubule asters are assumed to be important for regular spatial organization of the syncytial embryo. The functional interaction between the microtubule asters and cortical actin has been largely analyzed in a static manner, so far. We recorded the movement of centrosomes at 1 Hz and analyzed their fluctuations for two processes—pair separation and individual movement. We found that F-actin is required for directional movements during initial centrosome pair separation, because separation proceeds in a diffusive manner in latrunculin-injected embryos. For assaying individual movem...Continue Reading

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Citations

Apr 6, 2018·Journal of Cell Science·Anja Schmidt, Jörg Grosshans
Dec 6, 2017·PLoS Biology·Shuling YanJörg Großhans
Jan 24, 2018·Journal of Cell Science·Zhiyi LvJörg Großhans
Mar 22, 2020·Nature Communications·Gabriele SelvaggioSebastian Kruss
Feb 19, 2021·Journal of Cell Science·Zhiyi LvJörg Großhans
Oct 12, 2021·Journal of Cell Science·Anja SchmidtJörg Großhans

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Methods Mentioned

BETA
superresolution microscopy
transgenic

Software Mentioned

MATLAB
IMAGEJ

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