PMID: 11604214Oct 18, 2001Paper

Fluctuation of local points of F-actin sliding on the surface-fixed H-meromyosin molecules in the presence of ATP

Biophysical Chemistry
R Shimo, K Mihashi

Abstract

F-actin fragments fluorescently labeled with rhodamine-phalloidin were copolymerized with non-labeled F-actin fragments. F-actin copolymer consisted of several bright (fluorescent) and dark (non-fluorescent) stripes of approximately 1 microm in width. Local motion of individual speckled F-actin was investigated by measuring translocation fluctuation of several tracing points marked on the actin filament. The tracing points included the borders between neighboring bright and dark stripes, as well as the tip and tail of the filament. For speckled F-actin with an average sliding speed of 4.6 microm/s at 23 degrees C, the translocation distance of the tracing points (per 0.1 s) showed significant fluctuation, of the order of +/-0.12 microm/s, approximately 25% of the sliding speed. The fluctuation correlation of the translocation distance between two tracing points decreased as the distance between them increased. Statistical analysis of the correlation length of the translocation distance L(c) showed that L(c) increased with the sliding speed of the actin filament. The sliding speed, however, saturated as the correlation length became close to the persistence length of the bending elasticity of F-actin. On the contrary, the correl...Continue Reading

References

Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S J Kron, J A Spudich
Jun 20, 1969·Science·H E Huxley
Jan 1, 1974·Progress in Biophysics and Molecular Biology·T L Hill
Oct 22, 1971·Nature·A F Huxley, R M Simmons
Jan 15, 1980·Journal of Molecular Biology·H Nagashima, S Asakura
Nov 5, 1999·Biophysical Journal·J E BakerD D Thomas

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.