PMID: 7021594Aug 1, 1981Paper

Fluctuations of calcium, phosphorus, sodium, potassium, and chlorine in single alpha and beta cells during glucose perifusion of rat islets

The Journal of Clinical Investigation
R K Kalkhoff, K A Siegesmund

Abstract

To study the relationship between islet hormonal secretion and intracellular content of five elements, a rat islet perifusion technique was used in 24 paired experiments. Control and experimental chambers each containing 100 islets, received 2.8 and 16.7 mM D-glucose, respectively. Effluent was collected frequently for hormone measurements. At eight different time intervals form 0--30 min islets were fixed and prepared for scanning electron microscopy. Over 900 unobscured alpha and beta cells were selected by size and shape criteria. Energy dispersive x-ray analysis was applied to each single cell to determine relative content of calcium (Ca), potassium (K), sodium (Na), chlorine (Cl), and phosphorus (P). Experimental chambers exhibited typical acute (0--9 min) and second phase (10--30 min) insulin secretion in association with suppression of glucagon release after 10 min. At 2 min an abrupt upward K spike in both alpha and beta cells was followed at 3--4 min with a 1.5- to 2-fold rise of Ca and a reciprocal decrease in K, Na, Cl, and P. From 3 to 30 min biphasic insulin secretion. Reduced alpha cell calcium after 6 min preceded suppression of glucagon secretion. After 2 min K related inversely to Ca content in both alpha and b...Continue Reading

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Citations

Aug 7, 1992·Biochimica Et Biophysica Acta·M ErecińskaD Nelson
Mar 28, 1989·Biochimica Et Biophysica Acta·P LebrunA Herchuelz
Dec 1, 1986·Acta Physiologica Scandinavica·P BergstenB Hellman
May 1, 1988·Acta Physiologica Scandinavica·N Wesslén, B Hellman
Jun 28, 1985·Biochemical and Biophysical Research Communications·R C Johnson, N Freinkel
Feb 1, 1986·The American Journal of Physiology·T J BidenC B Wollheim

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