PMID: 16501812Feb 28, 2006Paper

Fludarabine induces apoptosis in chronic lymphocytic leukemia--the role of P53, Bcl-2, Bax, Mcl-1, and Bag-1 proteins

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas
J R FariaJ S R Oliveira

Abstract

The expression of P53, Bcl-2, Bax, Bag-1, and Mcl-1 proteins in CD5/CD20-positive B-chronic lymphocytic leukemia (B-CLL) cells from 30 typical CLL patients was evaluated before and after 48 h of incubation with 10(-6) M fludarabine using multiparametric flow cytometric analysis. Protein expression was correlated with annexin V expression, Rai modified clinical staging, lymphocyte doubling time, and previous treatment. Our main goal was to determine the predictive value of these proteins in CLL cells in terms of disease evolution. Bcl-2 expression decreased from a median fluorescence index (MFI) of 331.71 +/- 42.2 to 245.81 +/- 52.2 (P < 0.001) after fludarabine treatment, but there was no difference between viable cells (331.57 +/- 44.6 MFI) and apoptotic cells (331.71 +/- 42.2 MFI) before incubation (P = 0.859). Bax expression was higher in viable cells (156.24 +/- 32.2 MFI) than in apoptotic cells (133.56 +/- 35.7 MFI) before incubation, probably reflecting defective apoptosis in CLL (P = 0.001). Mcl-1 expression was increased in fludarabine-resistant cells and seemed to be a remarkable protein for the inhibition of the apoptotic process in CLL (from 233.59 +/- 29.8 to 252.04 +/- 35.5; P = 0.033). After fludarabine treatment,...Continue Reading

References

Nov 11, 1982·The New England Journal of Medicine·J J YunisA Theologides
Apr 22, 1996·International Journal of Cancer. Journal International Du Cancer·M Aguilar-SantelisesM Jondal
Jun 6, 2000·The Journal of Experimental Medicine·P C WilsonJ D Capra
Oct 12, 2000·Hematological Oncology·D P Bentley, C J Pepper
Nov 1, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S H Kaufmann, G J Gores
Feb 15, 2001·Molecular Aspects of Medicine·J S Bertram

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