Fluid dwell impact induces peritoneal fibrosis in the peritoneal cavity reconstructed in vitro

Journal of Artificial Organs : the Official Journal of the Japanese Society for Artificial Organs
Shigehisa AokiShuji Toda

Abstract

Peritoneal fluid dwell impacts the peritoneum by creating an abnormal physiological microenvironment. Little is known about the precise effects of fluid dwell on the peritoneum, and no adequate in vitro models to analyze the impact of fluid dwell have been established. In this study, we developed a peritoneal fluid dwell model combined with an artificial peritoneal cavity and fluid stirring generation system to clarify the effects of different dwelling solutions on the peritoneum over time. To replicate the peritoneal cavity, we devised a reconstructed peritoneal cavity utilizing a mesothelial layer, endothelial layer, and collagen membrane chamber. The reconstructed peritoneal cavity was infused with Dulbecco's modified Eagle's medium, saline, lactated Ringer's solution or peritoneal dialysis solution with repeated 4-h dwells for 10 or 20 consecutive days. The above-described solutions induced epithelial-mesenchymal transition (EMT) and hyperplasia of mesothelial cells. All solution types modulated nitric oxide synthase activities in mesothelial and endothelial cells and nitric oxide concentrations in dwelling solutions. Inhibition of nitric oxide synthase activity acted synergistically on mesothelial EMT and hyperplasia. The ...Continue Reading

References

Jan 1, 1997·Annual Review of Medicine·J P Cooke, V J Dzau
Mar 4, 1998·Arteriosclerosis, Thrombosis, and Vascular Biology·S JalaliJ Y Shyy
Nov 14, 2000·Circulation Research·H Cai, D G Harrison
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·D FukumuraR K Jain
May 17, 2001·Proceedings of the National Academy of Sciences of the United States of America·J SurapisitchatB C Berk
Mar 28, 2002·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Olivier Devuyst
Jan 31, 2003·The New England Journal of Medicine·María Yáñez-MóManuel López-Cabrera
Aug 6, 2003·American Journal of Physiology. Cell Physiology·Yong Chool Boo, Hanjoong Jo
Aug 26, 2004·Kidney International·Siska MortierAn S De Vriese
Sep 2, 2004·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Satoshi OgataNobuoki Kohno
Feb 23, 2008·Angiogenesis·Janice A NagyHarold F Dvorak
Oct 18, 2008·Journal of Pediatric Surgery·Alicja PołubinskaDimitrios G Oreopoulos
May 8, 2010·Journal of the American Society of Nephrology : JASN·Olivier DevuystNicholas Topley
May 3, 2011·Peritoneal Dialysis International : Journal of the International Society for Peritoneal Dialysis·Shigehisa AokiShuji Toda
Sep 6, 2011·European Heart Journal·Ulrich Förstermann, William C Sessa
Feb 22, 2012·Nature Reviews. Nephrology·Elvia García-LópezSimon Davies
Jul 23, 2013·Toxicological Sciences : an Official Journal of the Society of Toxicology·Hiroyuki YamaguchiToshiaki Takezawa
Sep 14, 2014·Clinical and Experimental Nephrology·Hiroyuki KadoyaNaoki Kashihara

❮ Previous
Next ❯

Citations

Aug 2, 2016·Pathology International·Shigehisa AokiShuji Toda
Feb 16, 2017·Journal of Artificial Organs : the Official Journal of the Japanese Society for Artificial Organs·Y SawaT Yamaoka
Apr 21, 2020·Therapeutic Apheresis and Dialysis : Official Peer-reviewed Journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy·Ji WangLining Miao

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.