Fluid dynamics during bleb formation in migrating cells in vivo

PloS One
Mohammad GoudarziErez Raz

Abstract

Blebs are cellular protrusions observed in migrating cells and in cells undergoing spreading, cytokinesis, and apoptosis. Here we investigate the flow of cytoplasm during bleb formation and the concurrent changes in cell volume using zebrafish primordial germ cells (PGCs) as an in vivo model. We show that bleb inflation occurs concomitantly with cytoplasmic inflow into it and that during this process the total cell volume does not change. We thus show that bleb formation in primordial germ cells results primarily from redistribution of material within the cell rather than being driven by flow of water from an external source.

References

Feb 9, 1996·Cell·D A Lauffenburger, A F Horwitz
Jan 1, 1996·Cell Motility and the Cytoskeleton·M YanaiS M Kelly
Feb 23, 2002·Trends in Cell Biology·J Victor SmallKlemens Rottner
Apr 8, 2004·Developmental Cell·Michal Reichman-FriedErez Raz
May 20, 2005·Nature·Guillaume T CharrasT J Mitchison
Oct 9, 2007·Biophysical Journal·Guillaume T CharrasL Mahadevan
Jun 11, 2008·The Journal of Cell Biology·Oliver T Fackler, Robert Grosse
Jul 17, 2008·Nature Reviews. Molecular Cell Biology·Guillaume Charras, Ewa Paluch
Aug 30, 2008·Journal of Microscopy·G T Charras
Jun 24, 2009·Nature Reviews. Molecular Cell Biology·Peter Friedl, Darren Gilmour
Oct 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jean-Yves TinevezEwa Paluch
Nov 17, 2009·Developmental Biology·Andy Aman, Tatjana Piotrowski
May 2, 2012·The Journal of Cell Biology·Ryan J PetrieKenneth M Yamada
Jun 19, 2012·Developmental Cell·Mehdi GoudarziErez Raz
Mar 13, 2014·The Journal of Cell Biology·Evgeny ZatulovskiyRobert R Kay
May 8, 2014·Development·Germán ReigMiguel L Concha
Jun 6, 2015·Physical Review Letters·Alessandro TaloniCaterina A M La Porta
Jan 20, 2016·The Journal of Cell Biology·Elena Scarpa, Roberto Mayor
Mar 2, 2016·Nature Cell Biology·Véronique GebalaHolger Gerhardt
Sep 4, 2016·BMC Biology·Alba Diz-MuñozEwa K Paluch
Aug 25, 2017·Scientific Reports·Aleix Boquet-PujadasAlexandre C Dufour
Oct 20, 2017·PLoS Genetics·Didier Y R StainierCecilia B Moens
Oct 28, 2017·Cell Research·Yingying ZhangJiahuai Han

❮ Previous
Next ❯

Citations

Jan 30, 2021·Patterns·Aleix Boquet-PujadasNancy Guillén
Feb 13, 2021·Proceedings of the National Academy of Sciences of the United States of America·Adan Olguin-OlguinErez Raz
Jul 13, 2021·Frontiers in Cell and Developmental Biology·Anne AaltoErez Raz

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
confocal microscopy

Software Mentioned

Visi
GraphPad Prism
View
Imaris
BioFlow
Excel
Visitron Systems
ICY

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme
K Ikenishi
Proceedings of the National Academy of Sciences of the United States of America
Jean-Yves TinevezEwa Paluch
© 2022 Meta ULC. All rights reserved