Fluid-induced lung injury-role of TRPV4 channels

Pflügers Archiv : European journal of physiology
Shailesh BihariAndrew D Bersten


Administration of bolus intravenous fluid is associated with respiratory dysfunction and increased mortality, findings with no clear mechanistic explanation. The objective of this study was to examine whether bolus intravenous (i.v.) fluid administration results in acute lung injury in a rat model and further, to examine whether this injury is associated with transient receptor potential vallinoid (TRPV)4 channel function and endothelial inflammatory response. Healthy male Sprague-Dawley rats were administered 60 ml/kg 0.9% saline i.v. over 30 min. Manifestation of acute lung injury was assessed by lung physiology, morphology, and markers of inflammation. The role of TRPV4 channels in fluid-induced lung injury was subsequently examined by the administration of ruthenium red (RR) in this established rat model and again in TRPV4 KO mice. In endothelial cell culture, permeability and P-selectin expression were measured following TRPV4 agonist with and without antagonist; 0.9% saline resulted in an increase in lung water, lavage protein and phospholipase A2, and plasma angiopoietin-2, with worsening in arterial blood oxygen (PaO2), lung elastance, surfactant activity, and lung histological injury score. These effects were ameliorat...Continue Reading


May 1, 1975·Journal of Applied Physiology·A L MuirH M Brash
Dec 9, 1976·The New England Journal of Medicine·J S ForresterH J Swan
Sep 1, 1990·American Journal of Respiratory Cell and Molecular Biology·T E NicholasH A Barr
May 1, 1973·Circulation·A P Fishman
Jan 1, 1972·Journal of Applied Physiology·J C HoggP T Macklem
Feb 1, 1967·Journal of Applied Physiology·N C StaubM L Pearce
May 9, 1998·Journal of Applied Physiology·J C ParkerJ A Tucker
Aug 17, 1999·The Journal of Clinical Investigation·W M KueblerJ Bhattacharya
Nov 15, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·K G DavidsonI R Doyle
Jan 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Landon S KingRobert H Brown
Apr 5, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Thalia Romani de WitJan A van Mourik
May 6, 2003·Journal of Applied Physiology·Riccardo PellegrinoVito Brusasco
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Oct 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Wolfgang Liedtke, Jeffrey M Friedman
Nov 22, 2005·Trends in Cardiovascular Medicine·Charles J LowensteinMunekazu Yamakuchi
Feb 8, 2006·Nature Medicine·Beat A Imhof, Michel Aurrand-Lions
May 6, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Ralf KöhlerJoachim Hoyer
Jul 22, 2006·Circulation Research·Ryuji InoueYushi Ito
Jul 31, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kazutoshi HamanakaJames C Parker
Sep 6, 2007·PloS One·Veronika HartmannsgruberRalf Köhler
Oct 27, 2007·American Journal of Respiratory Cell and Molecular Biology·Ming-Yuan JianMary I Townsley
Feb 29, 2008·Proceedings of the National Academy of Sciences of the United States of America·Venkataramana K SidhayeLandon S King
May 7, 2008·Pflügers Archiv : European journal of physiology·José M Fernández-FernándezMiguel A Valverde
May 24, 2008·The Journal of Pharmacology and Experimental Therapeutics·Robert N WilletteXiaoping Xu
Aug 23, 2008·Proceedings of the National Academy of Sciences of the United States of America·Ivan M LorenzoMiguel A Valverde
Apr 18, 2009·American Journal of Respiratory and Critical Care Medicine·Dani-Louise DixonAndrew D Bersten
Jun 18, 2009·The Journal of Biological Chemistry·Tsutomu MochizukiMakoto Tominaga
Oct 21, 2009·Cell Biochemistry and Biophysics·Jun Yin, Wolfgang M Kuebler
Aug 21, 2010·Integrative Biology : Quantitative Biosciences From Nano to Macro·Benjamin D MatthewsDonald E Ingber
Sep 28, 2010·American Journal of Respiratory and Critical Care Medicine·Claudia C dos dos SantosJack J Haitsma
May 28, 2011·The New England Journal of Medicine·Kathryn MaitlandUNKNOWN FEAST Trial Group
Feb 11, 2012·The American Journal of Pathology·Wai Y SunClaudine S Bonder
Mar 28, 2012·Analytical Biochemistry·Mingjiao SunYuehai Ke
May 23, 2012·Critical Care Medicine·Carolyn S CalfeeUNKNOWN NHLBI ARDS Network
Oct 19, 2012·The New England Journal of Medicine·John A MyburghUNKNOWN Australian and New Zealand Intensive Care Society Clinical Trials Group
Nov 9, 2012·Science Translational Medicine·Kevin S ThorneloeRobert N Willette
May 7, 2013·Proceedings of the National Academy of Sciences of the United States of America·Esther A SolymosiWolfgang M Kuebler
Oct 1, 2013·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Susanth V SukumaranSantosh Kumar Mishra
Nov 5, 2013·American Journal of Physiology. Heart and Circulatory Physiology·Edward A PankeyPhilip J Kadowitz

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Methods Mentioned

enzyme-linked immunosorbent assay
Protein Assay

Software Mentioned

C Flow Sampler

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