Fluid-phase marker transport in rat liver: free-flow electrophoresis separates distinct endosome subpopulations

Electrophoresis
Isabella EllingerR Fuchs

Abstract

Free-flow electrophoresis (FFE) was used to investigate the intracellular compartments involved in fluid-phase marker, fluoresceine isothiocyanate (FITC)-dextran, transport in the isolated perfused rat liver. One to 2 min after uptake at 37 degrees C, FITC-dextran was found in endosomes with the same electrophoretic mobility as early sorting endosomes labeled either by the hepatocyte-specific marker asialoorosomucoid (ASOR) or by transferrin that enters all liver cells. Labeling at low temperature (16 degrees C) blocked transport of ASOR and dextran in early endosomes. With increasing internalization time (3-13 min) at 37 degrees C, FITC-dextran-labeled compartments co-localized with late, ASOR-containing endosomes. Since localization of FITC-dextran in late transcytotic compartments was not observed upon FFE separation, it is concluded that the majority of internalized markers is directed to lysosomes. The FITC-label did not account for the predominant lysosomal targeting of the dextran, since [3H]dextran-labeled endosomes exhibited an identical FFE pattern. Taken together, these data indicate that the fluid-phase marker dextran is transported through intracellular compartments with identical characteristics as endosome subcom...Continue Reading

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Citations

Aug 29, 2006·Analytical and Bioanalytical Chemistry·Ryan D JohnsonEdgar A Arriaga
Aug 8, 2007·Analytical and Bioanalytical Chemistry·Ralf J BraunMarius Ueffing
Nov 11, 2008·Wiener medizinische Wochenschrift·Isabella Ellinger, Renate Fuchs
Feb 11, 2003·Cell Structure and Function·Maria de Nazaré Correia SoeiroMaria de Nazareth Leal Meirelles
Mar 30, 2010·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Shinji KakimotoTakeshi Nagasaki
Aug 7, 2009·American Journal of Physiology. Cell Physiology·Erick O Hernández-OchoaMartin F Schneider
Dec 8, 2020·American Journal of Physiology. Renal Physiology·Shiv Pratap S YadavMark C Wagner
Apr 13, 2006·Journal of the American Chemical Society·Christophe JungChristoph Bräuchle
Dec 22, 1998·European Journal of Cell Biology·J A SchmidP Kosma

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