Fluid shear of low magnitude increases growth and expression of TGFbeta1 and adhesion molecules in human bone cells in vitro

Experimental and Clinical Endocrinology & Diabetes : Official Journal, German Society of Endocrinology [and] German Diabetes Association
U M LiegibelC Kasperk

Abstract

Deformation of the bone matrix by mechanical strain causes fluid shifts within the osteocytic canaliculi which affect osteocytic cell metabolism. We applied low fluid shear (1 - 63 micro Pa for 10 - 48 h) to human osteoblastic cells (HOB) in vitro to study its impact on cell proliferation and differentiated functions. Proteins involved in translating the physical force into a cellular response were characterised. Low fluid shear stress stimulated proliferation of HOB 1.2-fold when stress was applied intermittently for 24 h. Shear stress also increased differentiated cellular properties such as alkaline phosphatase (ALP) activity (121 % of control), fibronectin (FN) and fibronectin receptor (FNR) expression (290 % and 200 %, respectively). Prostaglandin E (2) (PGE (2)) and TGFbeta1 release into the medium were significantly stimulated when shear stress was applied for 6 - 12 h and 24 - 48 h, respectively. TGFbeta1 + 2 neutralising antibodies or the presence of indomethacine inhibited the mitogenic effect of fluid shear and reduced ALP activity to its control level. Furthermore, TGFbeta treatment induced a dose-dependent increase in FN and FNR expression. Therefore, fluid shear stress of low magnitude (a) suffices to affect HOB m...Continue Reading

Citations

Jan 4, 2007·Journal of Materials Science. Materials in Medicine·Yifang CaoWole Soboyejo
Sep 23, 2003·Journal of Biomechanics·Liyun WangStephen C Cowin
Sep 21, 2011·Journal of Tissue Engineering and Regenerative Medicine·Beili ZhuC Mauli Agrawal
Jul 20, 2005·Biomaterials·E LeclercC Legallaisa
Dec 15, 2017·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Chengliang DengLu Feng
Jul 28, 2006·Nature·Jamil El-AliKlavs F Jensen
Dec 22, 2010·Tissue Engineering. Part C, Methods·Bertrand DavidHervé Petite
Jan 10, 2019·Journal of Cellular Physiology·Qinyu MaShiwu Dong
Sep 15, 2006·Journal of Cellular Physiology·Rebecca A JacksonSimon M Cool
Mar 7, 2007·Journal of Biomedical Materials Research. Part a·Z SchwartzB D Boyan
Aug 4, 2006·American Journal of Physiology. Cell Physiology·A F TaylorH J Donahue
Jan 21, 2014·Journal of Biomedical Materials Research. Part a·Juan XingYanfeng Luo
Aug 14, 2019·Periodontology 2000·Rafael Arcesio Delgado-RuizGeorgios E Romanos
Nov 30, 2016·Frontiers in Bioengineering and Biotechnology·Claudia WittkowskeCecile M Perrault
May 22, 2019·Lab on a Chip·Mary C RegierKelly R Stevens
Jun 18, 2019·Tissue Engineering and Regenerative Medicine·Morgan ChabanonBertrand David
Dec 2, 2020·Proceedings of the National Academy of Sciences of the United States of America·Manuel S BruggerChristoph Westerhausen
Apr 1, 2021·Acta Biomaterialia·Abdul Raouf AtifGemma Mestres

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