Fluid shear stress improves morphology, cytoskeleton architecture, viability, and regulates cytokine expression in a time-dependent manner in MLO-Y4 cells

Cell Biology International
Zedong YanDa Jing

Abstract

The effects of load-induced interstitial fluid shear stress (FSS) on instantaneous signaling response of osteocytes (e.g., calcium signaling) have been well documented. FSS can also initiate the release of many important messenger molecules of osteocytes (e.g., ATP and PGE2 ). However, the effects of FSS on cellular function and bone metabolism-modulating cytokine expression of osteocytes have not been fully identified (some inconsistent/conflicting results have been documented). Herein, osteocyte-like MLO-Y4 cells were stimulated with 1 Pa, 2-h FSS, and the effects of FSS on cellular morphology, cytoskeletal microstructure, biological activity, and gene and protein expression of important cytokines were investigated. SEM and cytoskeleton staining revealed that FSS induced well-organized cytoskeleton and increased filopodia processes. The osteocytic viability was sustained and apoptosis was inhibited via flow cytometry. FSS promoted Wnt3a and β-catenin gene and protein expression in 0-, 3-, and 6-h (sample collection time post FSS) groups. The FSS-stimulated cells in the 3-h group exhibited more significant effects on the promotion of OCN and Cx43 and inhibition of DKK1 and SOST expression than the 0- and 6-h groups. The 3-h gr...Continue Reading

References

Jul 14, 2004·Biochemical and Biophysical Research Communications·Astrid BakkerElisabeth Burger
Nov 5, 2004·Molecular Biology of the Cell·Joseph P Stains, Roberto Civitelli
May 4, 2006·The Journal of Clinical Investigation·Venkatesh KrishnanOrmond A Macdougald
Feb 16, 2007·Journal of Cellular Physiology·Damian C GenetosHenry J Donahue
Jul 25, 2007·Annals of the New York Academy of Sciences·Lynda F Bonewald
Feb 6, 2008·Journal of Cellular Physiology·Carmen E MacsaiCory J Xian
Feb 19, 2008·Bone·Lynda F Bonewald, Mark L Johnson
Jul 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Sirisha BurraJean X Jiang
Jul 27, 2010·Diabetic Medicine : a Journal of the British Diabetic Association·G MabilleauM E Edmonds
Jul 8, 2011·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Stacey M WooLynda F Bonewald
Sep 13, 2011·Nature Medicine·Tomoki NakashimaHiroshi Takayanagi
Feb 4, 2012·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·G J Atkins, D M Findlay
Feb 22, 2012·Critical Reviews in Eukaryotic Gene Expression·Peter S N Rowe
Feb 23, 2012·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Jinhu Xiong, Charles A O'Brien
Mar 7, 2012·Biochemical and Biophysical Research Communications·Rishikesh N KulkarniJenneke Klein-Nulend
Oct 23, 2012·Biochemical and Biophysical Research Communications·Ningyuan SunQuan Yuan
Feb 8, 2013·Nature Medicine·Roland Baron, Michaela Kneissel
Apr 10, 2013·Biomechanics and Modeling in Mechanobiology·Stefaan W VerbruggenLaoise M McNamara
Jul 23, 2013·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Erica L ClinkenbeardKenneth E White
Jul 24, 2013·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Xiaoting LiDing Zhang
Nov 1, 2013·Biomechanics and Modeling in Mechanobiology·Yoshitaka Kameo, Taiji Adachi
Dec 19, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Da JingX Edward Guo
Dec 21, 2013·Nature Reviews. Molecular Cell Biology·Peter E CzabotarJerry M Adams
Jan 22, 2014·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·M Á BrennanL M McNamara
Dec 23, 2014·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Marta MaycasPedro Esbrit
Jan 22, 2015·Proceedings of the National Academy of Sciences of the United States of America·Xiaolin TuTeresita Bellido
Feb 28, 2015·Biochemical and Biophysical Research Communications·Robert T BradyDavid A Hoey
Jun 6, 2015·Archives of Biochemistry and Biophysics·Minyi HuYi-Xian Qin
Aug 8, 2015·BioMed Research International·Rinaldo Florencio-SilvaPaulo Sérgio Cerri

❮ Previous
Next ❯

Citations

Sep 19, 2019·Biophysics Reviews·Roberta AlfieriFederica Viti
Feb 14, 2020·International Journal of Molecular Sciences·Manuel A RiquelmeJean X Jiang
Nov 6, 2020·Biomaterials Science·Kamol DeyLuciana Sartore
Feb 12, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·David A MonteiroTamara Alliston
Jul 4, 2021·Biochemical and Biophysical Research Communications·Xiao YangYu-Bo Fan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis