Fluid shear stress-induced nitric oxide production in human cavernosal endothelial cells: inhibition by hyperglycaemia.

BJU International
Hunter WessellsKanchan Chitaley

Abstract

To investigate whether fluid shear stress (FSS) induces endothelial nitric oxide synthase (eNOS) activity and NO production in isolated human corpus cavernosal endothelial cells (HCCECs), and whether this response is altered during hyperglycaemia in vitro, as haemodynamic signalling during penile erection induces eNOS-mediated NO production in vivo. ECs were cultured from HCC and characterized by the uptake of acetylated low-density lipoprotein and the expression of von Willebrand factor, VE-cadherin, CD31 and eNOS. HCCECs were exposed to FSS (1.2 Pa (12 dynes/cm2), 5 min) using a cone-and-plate viscometer in the presence or absence of high glucose (30 mm, 48 h). The phosphorylation of ser1177 on eNOS and total eNOS protein expression after FSS was examined by Western blot. NO in the conditioned media was assessed by measuring nitrate and nitrite levels. Compared to static conditions, FSS induced a significant increase in the phosphorylation of eNOS on ser1177 in HCCECs, and the release of NO to the conditioned media. Treatment of HCCECs with high glucose levels did not alter the ratio FSS-induced phosphorylated eNOS/total eNOS, but did result in the down-regulation of total eNOS and significantly attenuated FSS-induced NO rele...Continue Reading

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Citations

Nov 10, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Huihui DuanMinke Tang
Sep 7, 2014·Current Diabetes Reports·Konstantinos Hatzimouratidis, Dimitrios Hatzichristou
May 3, 2012·The Journal of Sexual Medicine·Olav SchjørringUlf Simonsen
Dec 11, 2008·The Journal of Sexual Medicine·Josephine Hidalgo-Tamola, Kanchan Chitaley
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Aug 6, 2015·Singapore Medical Journal·Hong LiuYongping Wu
Sep 7, 2012·Urology·Hongxiu NingChing-Shwun Lin
Nov 3, 2010·Metabolic Syndrome and Related Disorders·Bret G KelsoMichael R Caplan
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Aug 22, 2018·International Journal of Impotence Research·Parviz K KavoussiLisa A Palmer
Aug 17, 2018·Journal of Molecular and Cellular Cardiology·T D PaulaL M Bendhack

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