Abstract
To investigate whether fluid shear stress (FSS) induces endothelial nitric oxide synthase (eNOS) activity and NO production in isolated human corpus cavernosal endothelial cells (HCCECs), and whether this response is altered during hyperglycaemia in vitro, as haemodynamic signalling during penile erection induces eNOS-mediated NO production in vivo. ECs were cultured from HCC and characterized by the uptake of acetylated low-density lipoprotein and the expression of von Willebrand factor, VE-cadherin, CD31 and eNOS. HCCECs were exposed to FSS (1.2 Pa (12 dynes/cm2), 5 min) using a cone-and-plate viscometer in the presence or absence of high glucose (30 mm, 48 h). The phosphorylation of ser1177 on eNOS and total eNOS protein expression after FSS was examined by Western blot. NO in the conditioned media was assessed by measuring nitrate and nitrite levels. Compared to static conditions, FSS induced a significant increase in the phosphorylation of eNOS on ser1177 in HCCECs, and the release of NO to the conditioned media. Treatment of HCCECs with high glucose levels did not alter the ratio FSS-induced phosphorylated eNOS/total eNOS, but did result in the down-regulation of total eNOS and significantly attenuated FSS-induced NO rele...Continue Reading
References
Jul 1, 1997·Circulation·F CosentinoT F Lüscher
Jul 1, 1997·Arteriosclerosis, Thrombosis, and Vascular Biology·C Garlanda, E Dejana
Mar 21, 1998·Microvascular Research·L E CraigM C Zink
Oct 9, 1999·The Journal of Biological Chemistry·B GallisM A Corson
Apr 1, 2000·IEEE Transactions on Bio-medical Engineering·E Borowitz, O Barnea
Aug 23, 2000·The Journal of Urology·P HedlundK E Andersson
Feb 22, 2001·American Journal of Physiology. Renal Physiology·S V BrodskyM S Goligorsky
Aug 3, 2001·Urological Research·A StanariusU Jonas
Jan 16, 2002·Molecular Urology·A G Akingba, A L Burnett
Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·K Joseph HurtArthur L Burnett
Aug 21, 2002·Journal of Vascular Research·Ulf SimonsenDolores Prieto
Dec 17, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ingrid Fleming, Rudi Busse
Mar 20, 2003·The Journal of Biological Chemistry·Ian P SaltJohn R Petrie
Apr 11, 2003·The Journal of Urology·Trinity J BivalacquaWayne J G Hellstrom
Jul 11, 2003·The Journal of Urology·Karl-Erik Andersson
Sep 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jen-Tsan ChiPatrick O Brown
Feb 14, 2004·International Journal of Impotence Research·R ViragC Richard
Jun 30, 2004·International Journal of Impotence Research·A L Burnett
Oct 19, 2004·Diabetologia·S SrinivasanC C Hedrick
Aug 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Biljana MusickiArthur L Burnett
Citations
Jul 23, 2009·Physiological Genomics·Hunter WessellsKanchan Chitaley
Mar 15, 2013·International Journal of Impotence Research·X ChuA Agmo
Nov 10, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Huihui DuanMinke Tang
Sep 7, 2014·Current Diabetes Reports·Konstantinos Hatzimouratidis, Dimitrios Hatzichristou
Mar 17, 2007·Endothelium : Journal of Endothelial Cell Research·Paul ConnellPaul A Cahill
May 3, 2012·The Journal of Sexual Medicine·Olav SchjørringUlf Simonsen
Dec 11, 2008·The Journal of Sexual Medicine·Josephine Hidalgo-Tamola, Kanchan Chitaley
Feb 25, 2011·Microvascular Research·Peng Kai OngSangho Kim
May 12, 2010·Medical Hypotheses·Enchun LiJianglei Zhang
Jul 29, 2006·The Journal of Physiology·Nicole DuerrschmidtHenning Morawietz
Aug 30, 2007·The Journal of Pharmacy and Pharmacology·J L SartorettoZ B Fortes
Aug 6, 2015·Singapore Medical Journal·Hong LiuYongping Wu
Sep 7, 2012·Urology·Hongxiu NingChing-Shwun Lin
Aug 20, 2014·PloS One·Yong Sun ChoiSae Woong Kim
Nov 3, 2010·Metabolic Syndrome and Related Disorders·Bret G KelsoMichael R Caplan
Dec 18, 2015·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ryan L HoilandPhilip N Ainslie
Aug 22, 2018·International Journal of Impotence Research·Parviz K KavoussiLisa A Palmer
Aug 17, 2018·Journal of Molecular and Cellular Cardiology·T D PaulaL M Bendhack