PMID: 6089590Sep 1, 1984

Fluidity and composition of brush border and basolateral membranes from rat kidney

The American Journal of Physiology
M K HiseE J Weinman


The physical state of rat renal brush border and basolateral membranes was examined using the technique of electron spin resonance. As estimated from the order parameters of both 5- and 12-doxyl spin-labeled phosphatidylcholine, the brush border membrane was significantly more ordered than the basolateral membrane. To confirm these findings, fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene was studied. Fluorescence anisotropy at 25 and 37 degrees C was significantly greater in the brush border membrane when compared with the basolateral membrane. The relative contribution of the lipid component to this difference was examined using multilamellar liposomes prepared from lipid extracts of these membranes. Fluorescence anisotropy in the brush border membrane lipids was significantly greater than that in the basolateral membrane lipids at both 25 and 37 degrees C. Compositional determinants of fluidity were examined in both membranes. Factors that may contribute to the more fluid state of the basolateral membrane include 1) a greater lipid-to-protein ratio; 2) a greater ratio of phosphatidylcholine to sphingomyelin; and 3) a tendency toward shorter-length fatty acyl chains.


Oct 3, 2001·Physiological Reviews·J C HolthuisG Van Meer

Related Concepts

Body Fluids
Cyclic N-Oxides
Saturated Fat
Fluorescence Polarization
Cell Surface Proteins
Tissue Membrane

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.