Fluorescein as a Visible-Light-Induced Oxidase Mimic for Signal-Amplified Colorimetric Assay of Carboxylesterase by an Enzymatic Cascade Reaction

Chemistry : a European Journal
Li LiuHuzhi Zheng

Abstract

We have found that fluorescein possesses high visible-light-induced oxidase mimetic activity and could transform colorless 3,3',5,5'-tetramethylbenzidine (TMB) into blue oxidized TMB (oxTMB) without unstable and destructive H2 O2 under visible-light illumination. Instead, fluorescein uses oxygen as a mild and green electron acceptor, and its activity can be easily controlled by the switching "on/off" of visible light. In addition, the visible-light-induced catalytic mechanism was elucidated in detail and, as the main reactive species h+ and O2.- accounted for TMB oxidation. Based on the fact that fluorescein diacetate (FDA) possessed no activity and generated active fluorescein in situ in the presence of carboxylesterase (CaE), a signal-amplified sensing platform through a cascade reaction for CaE detection was constructed. Our proposed sensing system displayed excellent analytical performance for the detection of CaE in a wide linear range from 0.040 to 20 U L-1 with a low detection limit of 0.013 U L-1 . This work not only changes the conventional concept that fluorescein is generally considered to be photocatalytically inert, but also provides a novel sensing strategy by tailoring the enzyme mimetic activity of fluorescein d...Continue Reading

References

Jul 26, 2008·Nature Nanotechnology·Lizeng GaoXiyun Yan
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Nov 15, 2016·Chemical Communications : Chem Comm·Li LiuHuzhi Zheng
Feb 22, 2017·Chemistry : a European Journal·Chen WangXing-Hua Xia
Jun 14, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Meng GaoBin Liu

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Citations

Jun 5, 2019·Chemistry : a European Journal·Qingpu Wang, Oliver Steinbock
Aug 12, 2020·Analytical Methods : Advancing Methods and Applications·Liu ZhangXing Peng
Aug 28, 2020·Analytical and Bioanalytical Chemistry·Junren WangYi He
Jun 10, 2020·Luminescence : the Journal of Biological and Chemical Luminescence·Liu ZhangBaoxin Li
Aug 31, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Liu ZhangZixuan Zhang
May 29, 2019·Analytical Chemistry·Yaokun XiaJinghua Chen

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