PMID: 2105739Jan 16, 1990Paper

Fluorescence analysis of the size of a binding pocket of a peptide receptor at natural abundance

Biochemistry
Larry A SklarH Mueller

Abstract

We have studied the topography of interaction of a family of fluorescent formyl peptides containing four (CHO-Met-Leu-Phe-Lys-fluorescein), five (CHO-Met-Leu-Phe-Phe-Lys- fluorescein), and six (CHO-Nle-Leu-Phe-Nle-Tyr-Lys-fluorescein and CHO-Met-Leu-Phe-Phe-Phe-Lys- fluorescein) amino acids with their receptor using spectroscopic methods adapted to small sample volumes. Only the fluorescent peptides containing four and five amino acids were quenched upon binding to the receptor, indicating physical contact of the chromophore with the receptor. In contrast, only the hexapeptides were accessible to antibodies to fluorescein. Taken together, these results suggest that the carboxy terminus of the tetrapeptide or the pentapeptide is protected in the receptor binding pocket while the fluorescein on the carboxy terminus of either hexapeptide is exposed and recognized by the antibody to fluorescein. These results indicate that the binding pocket accommodates at least five but no more than six amino acids.

Citations

Jun 1, 1996·Research in Immunology·C R WangJ Deisenhofer
Jun 12, 2010·Journal of the American Chemical Society·Chutima Jiarpinitnun, Laura L Kiessling
Oct 26, 1999·The Journal of Peptide Research : Official Journal of the American Peptide Society·G CavicchioniS Spisani
Aug 31, 1999·Immunopharmacology and Immunotoxicology·M A Panaro, V Mitolo
Feb 1, 1994·Nuclear Medicine and Biology·P O ZamoraB A Rhodes
Sep 19, 2015·International Immunopharmacology·Igor A SchepetkinMark T Quinn
Sep 24, 2013·Biochimica Et Biophysica Acta·Rajashri SridharanMark E Dumont
Mar 11, 1999·Journal of Receptor and Signal Transduction Research·T L GilbertL A Sklar
Jul 4, 1998·Journal of Receptor and Signal Transduction Research·J C VilvenL A Sklar
Jan 1, 1996·Journal of Receptor and Signal Transduction Research·M D DomalewskiL A Sklar
Apr 18, 2015·Bioorganic & Medicinal Chemistry·Tomasz M Stepniewski, Slawomir Filipek
Sep 23, 1997·Biochemical and Biophysical Research Communications·O QuehenbergerR D Ye
Dec 13, 2017·Chembiochem : a European Journal of Chemical Biology·Anne C ConibearChristian F W Becker
Mar 25, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Simon LindHuamei Forsman
Nov 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Pangke YanMing-Wei Wang
Jul 9, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Erica L SouthgateRichard D Ye
Nov 29, 2013·The Journal of Biological Chemistry·Hui-Qiong HeRichard D Ye

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