Fluorescence and Förster resonance energy transfer investigations on DNA oligonucleotide and PAMAM dendrimer packing interactions in dendriplexes

Physical Chemistry Chemical Physics : PCCP
Hema Kumari Alajangi, Deenan Santhiya

Abstract

Considering the importance of short oligonucleotide packing in dendriplex-mediated gene delivery, a direct insight into the 14-mer oligonucleotide and dendrimer interactions using fluorescence and FRET techniques is the focus of this study. Fluorometric titrations of various fluorophore-tagged oligonucleotides with the first three PAMAM dendrimer generations showed a decrease in the fluorescence intensity with two break points, namely Z and Z, for each titration. The first break point for each dendrimer was identical to the neutralization point observed by basic biophysical studies for the corresponding dendrimer generations. Additionally, FRET studies on dual tagged oligonucleotide (DFT) molecules revealed a third break point at the charge ratio (Z) where there was the highest fluorescence energy transfer from the donor to the acceptor fluorophores. Altogether, dendriplex formation was considered to take place via three steps with an increase in the dendrimer concentration, where initially there was monomeric complexation at the neutralization point (Z) followed by loosely held molecular aggregation of the dendrimer (Z). In the final step, dendrimer molecular aggregates were held tightly together for the closest possible packi...Continue Reading

References

Aug 1, 1965·Journal of Molecular Biology·M J Waring
Mar 17, 2001·Nature Reviews. Genetics·S M HammondG J Hannon
Sep 17, 2004·Nature·Craig C Mello, Darryl Conte
May 24, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Koki WadaKaneto Uekama
Nov 29, 2005·Advanced Drug Delivery Reviews·Christine DufèsAndreas G Schätzlein
Jan 16, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Rong-Qin HuangChen Jiang
Apr 24, 2007·Advanced Drug Delivery Reviews·Lars Aagaard, John J Rossi
Jun 2, 2007·Nature Reviews. Drug Discovery·Antonin de FougerollesJudy Lieberman
Jun 26, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Dana J GaryYou-Yeon Won
Jun 28, 2008·The Journal of Physical Chemistry. B·Vaibhav M JadhavSouvik Maiti
Aug 12, 2008·Mini Reviews in Medicinal Chemistry·Yu GaoRong Qi
Aug 13, 2008·Journal of the American Chemical Society·Elina VuorimaaMarjo Yliperttula
Jul 2, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Oleh TaratulaTamara Minko
Jul 7, 2009·International Journal of Pharmaceutics·A P PerezM J Morilla
Nov 18, 2009·Biochemistry. Biokhimii︠a︡·D G ShcharbinM Bryszewska
Jan 21, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Christopher R SibleyMatthew J A Wood
Feb 12, 2010·The Journal of Physical Chemistry. B·Giovanni M PavanAndrea Danani
Apr 23, 2010·Science Translational Medicine·Anil PottiJoseph R Nevins
May 12, 2010·The Journal of Physical Chemistry. B·Marie-Louise AinalemTommy Nylander
Nov 3, 2010·Nanomedicine·Inmaculada PosadasValentín Ceña
Mar 23, 2011·International Journal of Pharmaceutics·Linda B JensenCamilla Foged
Jul 2, 2011·International Journal of Nanomedicine·Yan GaoXiao-Rong Li
Aug 30, 2011·Journal of Biomedical Nanotechnology·Surendra NimeshRamesh Chandra
Sep 21, 2011·Mutation Research·Itzel Sifuentes-RomeroAlejandra García-Gasca
Feb 11, 2012·Current Medicinal Chemistry·M JafariP Chen
Mar 21, 2012·Accounts of Chemical Research·Juliane Nguyen, Francis C Szoka
May 5, 2012·Cancer Gene Therapy·H ShenM Ferrari
Nov 28, 2013·Pharmaceuticals·Swati Biswas, Vladimir P Torchilin

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Citations

Nov 20, 2012·Biochemical and Biophysical Research Communications·Verena Rombach-RiegrafE M Galvez
Jul 12, 2005·Journal of Photochemistry and Photobiology. B, Biology·Yan-Jun HuSong-Sheng Qu

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