PMID: 9662158Jul 14, 1998Paper

Fluorescence and laser photon counting: measurements of epithelial [Ca2+]i or [Na+]i with ciliary beat frequency

Annals of Biomedical Engineering
Hua Mao, L B Wong

Abstract

We describe a system we developed that enabled simultaneous measurements of either epithelial calcium ion concentration ([Ca2+]i) or sodium ion concentration ([Na+]i) with the ciliary beat frequency (CBF) in native ciliated epithelia using either Fura-2 (AM) or SBFI (AM) ratiometric fluorescence photon counting along with nonstationary laser light scattering. Studies were performed using native epithelial tissues obtained from ovine tracheae. The dynamic range of the laser light-scattering system was determined by a simulated light "beating" experiment. The nonstationary CBF was demonstrated by the time-frequency analysis of the raw photon count sequences of backscattered heterodyne photons from cultured and native epithelia. Calibrations of calcium and sodium ion concentrations were performed using the respective Fura-2 and SBFI impermanent salts as well as in native epithelia. The cumulative responses of 10(-6), 10(-5), and 10(-4) M nifedipine on [Ca2+]i together with the CBF as well as the cumulative responses of 10(-5), 10(-4), and 10(-3) M amiloride on [Na+]i together with the CBF were also determined. Nifedipine decreased [Ca2+]i but had no effect on CBF. Amiloride decreased [Na+]i and CBF. Stimulation of CBF corresponded...Continue Reading

Citations

Nov 27, 2002·The Journal of General Physiology·Weiyuan MaZvi Priel
Dec 3, 2009·Tissue Engineering. Part C, Methods·Yuchi WangHua Mao
Apr 27, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jae-Kyun KoJianjie Ma
Mar 12, 2008·Biochimica Et Biophysica Acta·Liubov Lemberskiy-KuzinZvi Priel

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