Fluorescence anisotropy in indole under two-photon excitation in the spectral range 385-510 nm

Physical Chemistry Chemical Physics : PCCP
M E SasinO S Vasyutinskii

Abstract

This paper presents the detailed study of two-photon excited fluorescence in indole dissolved in propylene glycol produced by two-photon absorption from the molecular ground state to several high lying excited states. The experimental method involved excitation with linearly and circularly polarized femtosecond pulses and time-resolved detection of the polarized fluorescence decay. The fluorescence intensity, anisotropy, excited state lifetime, and rotation diffusion time as function of the excitation light wavelength in the spectral range 385-510 nm were determined in experiment. The theoretical fit of the experimental results obtained demonstrated the contributions of six highly excited molecular states of different symmetry to the two-photon absorption intensity and fluorescence anisotropy. An intense two-photon absorption peak was observed experimentally in the spectral range 385-480 nm and explained as contributions from four high lying electronic excited states. The temporal dependence of fluorescence intensity in indole was satisfactory characterized by a single excited state lifetime τf and a single rotational diffusion time τrot. As shown, the excited state lifetime τf depends on the excitation light wavelength, which ...Continue Reading

References

Jan 1, 1997·Annual Review of Physical Chemistry·P R Callis
Aug 4, 2007·The Journal of Chemical Physics·Vladislav V Kuznetsov, Oleg S Vasyutinskii
Dec 17, 2008·Analytical Biochemistry·Henryk SzmacinskiJoseph R Lakowicz
Aug 1, 2009·The Journal of Physical Chemistry. a·Gabriel G Balint-Kurti, Oleg S Vasyutinskii
Aug 13, 2009·Physical Chemistry Chemical Physics : PCCP·Oleg OpanasyukLennart B-A Johansson
Apr 21, 2010·Physical Chemistry Chemical Physics : PCCP·Jochen KüpperMichael Schmitt
Apr 23, 2010·Physical Chemistry Chemical Physics : PCCP·Christian BrandMichael Schmitt
Jan 17, 2015·The Journal of Chemical Physics·Sebastian HerbrichOleg S Vasyutinskii

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Citations

Aug 9, 2020·Physical Chemistry Chemical Physics : PCCP·Ioanna A GorbunovaOleg S Vasyutinskii
Jul 19, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Dina M BeltukovaOleg S Vasyutinskii
Nov 12, 2020·The Journal of Physical Chemistry. B·Ioanna A GorbunovaOleg S Vasyutinskii

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