Fluorescence assay for alkaline phosphatase based on ATP hydrolysis-triggered dissociation of cerium coordination polymer nanoparticles

The Analyst
Chuanxia ChenYizhong Lu

Abstract

Alkaline phosphatase (ALP) is a significant biomarker for diagnostics. Simple, selective and sensitive detection of ALP activity is thus of critical importance. In this study, an artful fluorescence assay for ALP is proposed based on adenosine triphosphate (ATP) hydrolysis-triggered disassociation and fluorescence quenching of cerium coordination polymer nanoparticles (CPNs). ATP, a recognized natural substrate of phosphatase, can serve as a superb "antenna" to sensitize the luminescence of Ce3+ with the aid of tris(hydroxymethyl) aminomethane (Tris), forming Ce3+-ATP-Tris CPNs. In the presence of ALP, ATP will be catalytically converted into adenosine and inorganic orthophosphate, however neither of them can sensitize Ce3+ in alkaline media. As a result, the obtained CPNs are disassociated, inducing the quenching of the fluorescence. On this basis, a straightforward fluorescence assay for ALP activity is rationally developed. The fluorescence quenching efficiency shows a linear relationship for ALP within the activity range from 0.1 to 10 mU mL-1 with a detection limit of 0.09 mU mL-1 under the optimal experimental conditions. Moreover, this facile yet effective fluorescence method featured simplicity, cost-effectiveness, high...Continue Reading

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Citations

Sep 10, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Hongwei SongXiangheng Niu
Apr 3, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xilu HuMin Wang
Sep 19, 2019·Dalton Transactions : an International Journal of Inorganic Chemistry·Mei-Yu LiangYan Li
Dec 23, 2020·Dalton Transactions : an International Journal of Inorganic Chemistry·Tumpa GoraiThorfinnur Gunnlaugsson
Nov 5, 2019·Biosensors & Bioelectronics·Yangxia HanHongdeng Qiu
Aug 11, 2021·Chembiochem : a European Journal of Chemical Biology·Tal GilboaDavid R Walt

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Methods Mentioned

BETA
fluorescence assay
Fluorescence
biosensor
enzyme assay

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