Fluorescence assays for high-throughput screening of protein kinases

Combinatorial Chemistry & High Throughput Screening
Guido J R ZamanC A A van Boeckel

Abstract

Protein kinases comprise one of the most important group of targets for drug discovery research today. Methods to identify novel kinase inhibitors by high-throughput screening have evolved rapidly in recent years. An important aspect is the availability of fluorescent probes that can be applied in a homogeneous, or mix-and-measure, assay format. Here, we illustrate the application of fluorescence read-out technologies for kinase targets in light of our own experiences in assay development and high-throughput screening.

Citations

Mar 23, 2005·Nature Methods·Benjamin E Turk
Feb 25, 2005·Nature Reviews. Drug Discovery·Frank E Koehn, Guy T Carter
Jul 10, 2012·Molecular BioSystems·Guangchang ZhouStephen J Kron
Feb 4, 2009·Assay and Drug Development Technologies·Karen M Kleman-LeyerRobert G Lowery
Jan 31, 2006·Expert Opinion on Therapeutic Targets·Robert G Lowery, Karen Kleman-Leyer
Dec 1, 2008·Expert Opinion on Drug Discovery·Yong Jia
Aug 1, 2008·Expert Opinion on Drug Discovery·Yong JiaMarkus Warmuth
Feb 14, 2012·Expert Opinion on Drug Discovery·Wendy A Lea, Anton Simeonov
Aug 19, 2015·Biotechnology Advances·Atanas G AtanasovHermann Stuppner
Jan 16, 2016·Journal of Peptide Science : an Official Publication of the European Peptide Society·T Amanda StromAndrew R Barron
Apr 9, 2008·Trends in Pharmacological Sciences·Didier Leroy, Christian Doerig
Mar 19, 2008·Chembiochem : a European Journal of Chemical Biology·Ivan PartserniakJohn D Brennan
Dec 21, 2013·Biotechnology Journal·Camille PrévelMay C Morris
Dec 5, 2006·Journal of Pharmacological and Toxicological Methods·Matt DeaconChristine Williams
Apr 2, 2005·Analytical Biochemistry·Kaido VihtAsko Uri
Oct 16, 2014·European Journal of Medicinal Chemistry·Camille PrévelMay C Morris
May 5, 2004·Chemistry & Biology·Jayaram SrinivasanJohn L Diener
Jan 7, 2005·Journal of Biomolecular Screening·Achim Von LeoprechtingFrank H Büttner
May 23, 2006·Assay and Drug Development Technologies·Dominique PerrinAlexander Scheer
Jun 6, 2006·Journal of Biomolecular Screening·Dominique PerrinAlexander Scheer
Jun 6, 2006·Journal of Biomolecular Screening·Neil W CharterRichard M Eglen
May 29, 2004·Assay and Drug Development Technologies·James R BeasleyDavid A Dunn
May 29, 2004·Assay and Drug Development Technologies·Mitsunori Koresawa, Takayoshi Okabe
May 29, 2004·Assay and Drug Development Technologies·Pirthipal SinghPeter M Broad
Jan 29, 2010·Assay and Drug Development Technologies·Jasbinder SangheraJun Yan
Jan 29, 2010·Assay and Drug Development Technologies·Hicham ZegzoutiSaid A Goueli
Dec 14, 2006·ACS Chemical Biology·Michael D AllenJin Zhang

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