Fluorescence-based measurement of store-operated calcium entry in live cells: from cultured cancer cell to skeletal muscle fiber.

Journal of Visualized Experiments : JoVE
Zui PanMarco Brotto

Abstract

Store operated Ca(2+) entry (SOCE), earlier termed capacitative Ca(2+) entry, is a tightly regulated mechanism for influx of extracellular Ca(2+) into cells to replenish depleted endoplasmic reticulum (ER) or sarcoplasmic reticulum (SR) Ca(2+) stores. Since Ca(2+) is a ubiquitous second messenger, it is not surprising to see that SOCE plays important roles in a variety of cellular processes, including proliferation, apoptosis, gene transcription and motility. Due to its wide occurrence in nearly all cell types, including epithelial cells and skeletal muscles, this pathway has received great interest. However, the heterogeneity of SOCE characteristics in different cell types and the physiological function are still not clear. The functional channel properties of SOCE can be revealed by patch-clamp studies, whereas a large body of knowledge about this pathway has been gained by fluorescence-based intracellular Ca(2+) measurements because of its convenience and feasibility for high-throughput screening. The objective of this report is to summarize a few fluorescence-based methods to measure the activation of SOCE in monolayer cells, suspended cells and muscle fibers. The most commonly used of these fluorescence methods is to direc...Continue Reading

References

May 4, 2002·Nature Cell Biology·Zui PanJianjie Ma
Nov 7, 2002·The Journal of Biological Chemistry·Dong Wook ShinJianjie Ma
Nov 29, 2002·Frontiers in Bioscience : a Journal and Virtual Library·Jianjie Ma, Zui Pan
Feb 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Bradley S LaunikonisD George Stephenson
May 27, 2003·Cell Calcium·Jianjie Ma, Zui Pan
Mar 25, 2005·Physiological Reviews·Anant B Parekh, James W Putney
Apr 17, 2010·Nature Reviews. Drug Discovery·Anant B Parekh

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