Fluorescence-based sorting of neural stem cells and progenitors

Current Protocols in Neuroscience
Dragan Maric, Jeffery L Barker

Abstract

Neural stem cells (NSCs) are defined as undifferentiated cells originating from the neuroectoderm that have the capacity both to perpetually self-renew without differentiating and to generate multiple types of lineage-restricted progenitors (LRPs). LRPs can themselves undergo limited self-renewal and ultimately differentiate into highly specialized cells that make up the nervous system. However, this physiologically delimited definition of NSCs and LRPs has become increasingly blurred due to lack of protocols for effectively separating these types of cells from primary tissues. This unit discusses recent attempts using fluorescence-activated cell sorting (FACS) strategies to prospectively isolate NSCs from different types of LRPs as they appear in vivo, and details a protocol that optimally attains this goal. Thus, the strategy presented here provides a framework for more precise studies of NSC and LRP cell biology in the future, which can be applied to all vertebrates, including humans.

References

Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A S Blum, C J Barnstable
Jul 1, 1988·Science·G J SpangrudeI L Weissman
Oct 1, 1988·Journal of Neuroscience Research·T BeharM Dubois-Dalcq
Oct 24, 1983·Brain Research·N Kasai, R K Yu
Nov 15, 1983·Biochemical and Biophysical Research Communications·S K KunduD M Marcus
Dec 1, 1983·Developmental Biology·A KoulakoffY Berwald-Netter
Jan 1, 1982·The Journal of Membrane Biology·P H Fishman
Feb 1, 1993·Molecular Microbiology·J M Rolf, L Eidels
Apr 1, 1996·The Journal of Experimental Medicine·M A GoodellR C Mulligan
Apr 30, 1996·Brain Research. Developmental Brain Research·K S Shindler, K A Roth
May 1, 1999·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·S M KraggerudS D Fossa
Nov 24, 1999·Brain Research. Developmental Brain Research·T A McIntyreI A Shibley
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·N UchidaI L Weissman
Feb 17, 2001·Molecular and Cellular Neurosciences·A KawaguchiH Okano
Mar 29, 2001·Molecular and Cellular Neurosciences·M F SantiagoR Mendez-Otero
Apr 27, 2001·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·L M MiyakoshiC Hedin-Pereira
Nov 1, 2001·Annual Review of Cell and Developmental Biology·I L WeissmanF Gage
Nov 2, 2001·Nature·S Temple
Jul 9, 2002·Current Opinion in Genetics & Development·David M Panchision, Ronald D G McKay
Nov 19, 2002·Developmental Biology·Jingli CaiMahendra S Rao
Feb 20, 2003·Trends in Neurosciences·Raewyn M Seaberg, Derek van der Kooy
Jul 10, 2003·Trends in Neurosciences·Larysa Pevny, Mahendra S Rao
Mar 17, 2004·Current Opinion in Neurobiology·Cindi M Morshead, Derek van der Kooy
Apr 15, 2004·Molecular and Cellular Neurosciences·M A HackM Götz
Jul 13, 2004·Molecular Neurobiology·Dragan Maric, Jeffery L Barker
Sep 16, 2005·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Mack Fulwyler

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.