Fluorescence change of Fusobacterium nucleatum due to Porphyromonas gingivalis

The Journal of Microbiology
Min-Ah LeeSeung-Hwa Jeong

Abstract

The aim of this study was to measure changes in the fluorescence of Fusobacterium nucleatum interacting with Porphyromonas gingivalis for excitation with blue light at 405-nm. P. gingivalis was mono- and co-cultivated in close proximity with F. nucleatum. The fluorescence of the bacterial colonies was photographed using a QLF-D (Quantitative Light-induced Fluorescence-Digital) Biluminator camera system with a 405 nm light source and a specific filter. The red, green and blue intensities of fluorescence images were analyzed using the image analysis software. A fluorescence spectrometer was used to detect porphyrin synthesized by each bacterium. F. nucleatum, which emitted green fluorescence in single cultures, showed intense red fluorescence when it was grown in close proximity with P. gingivalis. F. nucleatum co-cultivated with P. gingivalis showed the same pattern of fluorescence peaks as for protoporphyrin IX in the red part of the spectrum. We conclude that the green fluorescence of F. nucleatum can change to red fluorescence in the presence of adjacent co-cultured with P. gingivalis, indicating that the fluorescence character of each bacterium might depend on the presence of other bacteria.

References

Apr 1, 1997·Advances in Dental Research·P D Marsh, D J Bradshaw
May 16, 2002·Periodontology 2000·Sigmund S Socransky, Anne D Haffajee
Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Paul E KolenbranderRobert J Palmer
Sep 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Michiko E Taga, Bonnie L Bassler
Mar 29, 2005·Antimicrobial Agents and Chemotherapy·Nikolaos S SoukosJ Max Goodson
Dec 15, 2005·Caries Research·A M LennonT Attin
Oct 26, 2006·Caries Research·M H van der VeenJ J de Soet
Jun 2, 2010·Nature Reviews. Microbiology·Paul E KolenbranderNicholas S Jakubovics
Jul 23, 2011·Virulence·Ruijie HuangRichard L Gregory
May 11, 2013·European Journal of Oral Sciences·Catherine M C VolgenantJacob M ten Cate
Oct 12, 2013·Archives of Oral Biology·Yun-Ji JangBong-Kyu Choi
Jul 15, 2015·Analytical and Bioanalytical Chemistry·Jonas FyrestamConny Östman
Dec 23, 2015·Photodiagnosis and Photodynamic Therapy·Sun-Young HanBaek-Il Kim
Feb 28, 2016·Journal of Dentistry·Monique H van der VeenWim Crielaard
Apr 10, 2016·Journal of Oral Microbiology·Catherine M C VolgenantMonique H van der Veen

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