Fluorescence correlation spectroscopy

Methods in Molecular Biology
Kirsten Bacia, Petra Schwille

Abstract

Fluorescence correlation spectroscopy (FCS) is a technique that allows for an extremely sensitive determination of molecular diffusion properties, down to the level of single molecules. It thus provides an attractive alternative to FRAP, requiring much less laser power and lower concentrations of fluorophores. FCS has recently been applied on live cells, and in comparison on domain-forming model membrane systems, to systematically study the influence of cholesterol on local membrane structure by investigating the mobility of selected lipid probes. The findings demonstrate the ability of FCS to sensitively distinguish between different local lipid structures, and emphasize the value of model systems for understanding membrane dynamics in general.

Citations

Feb 12, 2010·Proceedings of the National Academy of Sciences of the United States of America·Vladana VukojevicRudolf Rigler
Jul 28, 2011·Molecular and Cellular Biology·Mohsen Karimi ArzenaniTomas J Ekström
Jan 12, 2011·Annual Review of Physical Chemistry·Michelle A Digman, Enrico Gratton
Dec 23, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Chilbert DongMarianna Foldvari
Jul 1, 2010·Optics Express·Daniel BoeningJurgen Klingauf
Nov 17, 2007·Nature Protocols·Kirsten Bacia, Petra Schwille
Mar 15, 2012·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Michelle A Digman, Enrico Gratton
Jan 10, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Annelies GeeraertsYves Engelborghs
Dec 18, 2013·Chemical Society Reviews·Priya R Banerjee, Ashok A Deniz
Jan 3, 2013·Angewandte Chemie·Matthias SchulzWolfgang H Binder
Feb 19, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Charlisa R DanielsChristy F Landes
Aug 26, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Vladana VukojevićLudmilla A Morozova-Roche
Dec 2, 2008·Biochimica Et Biophysica Acta·Charles A Day, Anne K Kenworthy

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