Jul 27, 1976

Fluorescence decay studies of reduced nicotinamide adenine dinucleotide in solution and bound to liver alcohol dehydrogenase

Biochemistry
A Gafni, L Brand

Abstract

The monophoton counting technique was used to obtain the fluorescence decay kinetics of NADH (dihydronicotinamide adenine dinucleotide) bound to LADH (HORSE LIVER ALCOHOL DEHYDROGENAS). It was found that the fluorescence decay of the enzyme complex did not follow a single exponential decay law but that the data could be well described as a sum of two exponentials. The decay parameters of the enzyme complex do not depend on the degree of binding-site saturation. These results are interpreted in terms of a reversible excited-state reaction forming a nonfluorescent product. Fluorescence decay kinetics are also reported for NADH and related molecules in solution. The decay parameters, fluorescence emission maxima, and fluorescence intensities depend on solvent polarity and viscosity.

Mentioned in this Paper

Alcohol Oxidoreductases
NADH
Fluorescence Spectroscopy
Plasma Protein Binding Capacity
ADH1A
Alcohol dehydrogenase
Nicotinamide adenine dinucleotide (NAD)
Blood Viscosity Examination
Hydrogen-Ion Concentration
Disintegration (Morphologic Abnormality)

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