Fluorescence Detection of Bone Microcracks Using Monophosphonated Carbon Dots

ACS Applied Materials & Interfaces
Fatemeh OstadhosseinDipanjan Pan

Abstract

Phosphonated compounds, in particular, bisanalogs are widely applied in clinical settings for the treatment of severe bone turnovers and recently as imaging probes when conjugated with organic fluorophores. Herein, we introduce a bone seeking luminescent probe that shows a high binding affinity toward bone minerals based on monophosphonated carbon dots (CDs). Spheroidal CDs tethered with PEG monophosphates are synthesized in a one-pot hydrothermal method and are physicochemically characterized, where the retention of phosphonates is confirmed by 13P NMR and X-ray photoelectron spectroscopy. Interestingly, the high abundance of multiple monodentate phosphonates exhibited strong binding to hydroxyapatite, the main bone mineral constituent. The remarkable optophysical properties of monophosphonated CDs were confirmed in an ex vivo model of the bovine cortical bone where the imaging feasibility of microcracks, which are calcium-rich regions, was demonstrated. The in vivo studies specified the potential application of monophosphonated CDs for imaging when injected intramuscularly. The biodigestible nature and cytocompatibility of the probe presented here obviate the demand for a secondary fluorophore, while offering a nanoscale stra...Continue Reading

References

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Jul 28, 2016·Proceedings of the National Academy of Sciences of the United States of America·Qian YinTimothy M Fan
Aug 23, 2016·Small·Fatemeh OstadhosseinDipanjan Pan
Oct 30, 2016·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Fatemeh Ostadhossein, Dipanjan Pan
Mar 14, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Evandro M AlexandrinoFrederik R Wurm

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Citations

Jan 25, 2020·Advanced Healthcare Materials·Zhili PengRoger M Leblanc
Jan 28, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Anqi LiJianshu Li
Feb 8, 2020·ACS Applied Materials & Interfaces·Fatemeh OstadhosseinDipanjan Pan

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