Abstract
The diagnostic depth of photodynamic diagnosis (PDD) for gastric cancer with protoporphyrin IX (PpIX) is limited, which leads to missing intramucosal cancers in screening and surgery. The reason is that the excitation light, whose wavelength is determined by the highest absorption peak of PpIX (∼405 nm), is strongly attenuated by mucosal tissues. We investigated an excitation wavelength that can extend the diagnostic depth of PpIX fluorescence at the mucosal subsurface. By calculating the depth-dependent intensity of the excitation light in porcine gastric mucosa for each wavelength, relationships among the wavelength, fluorophore depth, and fluorescence intensity were assessed and fluorescence images of PpIX pellets located at different fluorophore depths were compared experimentally by changing the excitation wavelength. The numerical calculation showed that a 505-nm excitation light provided the highest fluorescence intensities at a fluorophore depth deeper than 1.1 mm. In the fluorescence observation, the fluorescence intensities at fluorophore depths of 0 and 1.0 mm at 405 nm were 5.4 × 103 and 1.0 × 103 arb. units, whereas those at 505 nm were 5.3 × 101 and 1.9 × 102 arb. units, respectively. The experimental r...Continue Reading
References
Oct 1, 1991·Radiology·H FujishimaH Nawata
Sep 1, 1993·Journal of Photochemistry and Photobiology. B, Biology·C S LohS B Brown
Oct 25, 2000·Photochemistry and Photobiology·M C AaldersN van der Vange
Dec 1, 2001·Journal of Biomedical Optics·R DrezekR Richards-Kortum
May 3, 2008·Physics in Medicine and Biology·E Salomatina, A N Yaroslavsky
Feb 1, 1993·Applied Optics·I F Cilesiz, A J Welch
Jan 5, 2011·Journal of Biomedical Optics·Anthony KimBrian C Wilson
Feb 20, 2013·Journal of Gastroenterology·Masaaki KobayashiYutaka Aoyagi
Oct 15, 2013·Lasers in Medical Science·Nienke BosschaartDirk J Faber
Nov 12, 2013·Journal of Surgical Oncology·Tsutomu NamikawaKazuhiro Hanazaki
Aug 21, 2014·Oncology·Kentaro KishiMasato Sakon
Mar 11, 2015·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Akiko SakaSatoshi Nimura
Aug 14, 2015·World Journal of Gastroenterology : WJG·Tsutomu NamikawaKazuhiro Hanazaki
Oct 2, 2015·Digestive Diseases and Sciences·Kazutoshi HoriHiroto Miwa
Dec 15, 2015·Journal of Biomedical Optics·Norihiro HondaKunio Awazu
Jan 27, 2016·World Journal of Gastroenterology : WJG·Noriaki KoizumiTetsuro Takamatsu
Feb 21, 2016·Digestive Endoscopy : Official Journal of the Japan Gastroenterological Endoscopy Society·Manabu MutoHisao Tajiri
Nov 4, 2016·Surgery Today·Kentaro KishiMasato Sakon
Aug 5, 2017·Journal of Neurosurgery·David W RobertsKeith D Paulsen