Fluorescence Differentiation of ATP-related Multiple Enzymatic Activities in Synovial Fluid as a Marker of Calcium Pyrophosphate Deposition Disease using Kyoto Green

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Nattha YongwattanaJirarut Wongkongkatep

Abstract

Calcium pyrophosphate deposition disease (CPPD) is a crystal induced inflammation in joints, and causes severe pain in elderly people. The accumulation of pyrophosphate (PPi) in synovial fluid (SF) results from several enzymatic reactions, especially the highly activated e-NPPs, which catalyze the conversion of ATP to PPi. This study demonstrates the detection of relative catalytic activity of 3 enzymes-ecto-nucleotide pyrophosphatase/phosphodiesterases (e-NPPs), tissue nonspecific alkaline phosphatase (TNAP), and ecto-nucleoside triphosphate diphosphohydrolases (e-NTPDases)-using a single molecular sensor called Kyoto Green. Kyoto Green exhibits excellent performance in sensing the catalytic activity of the commercial representatives of the e-NPPs, TNAP, and e-NTPDases, which are ENPP1, PPase, and apyrase, respectively, in both single-enzyme and multi-enzyme assays. Analysis of SF enzymes in 19 SF samples from human and swine revealed moderate activity of e-NPPs, high activity of e-NTPDases, and low activity of TNAP. Our newly developed method for analysis of multiple enzymatic activities using Kyoto Green in biological SF will assist improvement in accuracy of the CPPD prognosis/diagnosis, which will minimize unnecessary medi...Continue Reading

References

Dec 1, 1985·Arthritis and Rheumatism·J W Rachow, L M Ryan
Jul 21, 2000·Arthritis and Rheumatism·R D GraffG M Lee
Apr 23, 2003·Current Opinion in Rheumatology·Ronald D GraffGreta M Lee
Aug 30, 2005·Trends in Biochemical Sciences·Cristiana StefanMathieu Bollen
Apr 19, 2007·Chemistry, an Asian Journal·Akio OjidaItaru Hamachi
Aug 15, 2008·Journal of the American Chemical Society·Akio OjidaItaru Hamachi
May 5, 2012·Purinergic Signalling·Herbert ZimmermannNorbert Sträter
Dec 12, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Sang-Yong LeeChrista E Müller
Sep 12, 2013·The Journal of Biological Chemistry·Mark I MelhornIonita C Ghiran
Nov 30, 2013·Arthritis Research & Therapy·Ann K RosenthalLawrence M Ryan
May 3, 2014·Accounts of Chemical Research·Garima Ghale, Werner M Nau
Jun 30, 2016·The New England Journal of Medicine·Ann K Rosenthal, Lawrence M Ryan
Dec 25, 2016·Biochimica Et Biophysica Acta. General Subjects·Vigneshwaran NamasivayamChrista E Müller
Mar 3, 2017·Topics in Current Chemistry·Jirarut WongkongkatepItaru Hamachi
Mar 7, 2017·Frontiers in Pharmacology·Sang-Yong LeeChrista E Müller
Oct 3, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jingjing ZhangYong Chen
Aug 15, 2019·Chemical Communications : Chem Comm·Chaoqun WangYi Lv
Oct 3, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Hui HuangYongxin Li

❮ Previous
Next ❯

Methods Mentioned

BETA
nucleic acid amplification
PPase

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.