Fluorescence enhancement and multiple protein detection in ZnO nanostructure microfluidic devices

Biosensors & Bioelectronics
Chen-Hsiang SangJeng-Tzong Sheu

Abstract

In this study, different morphological ZnO nanostructures, those of sharp nanowires (NWs), rod NWs, and hexahedral-puncheon nanostructures, were grown in microfluidic channels on the same glass substrate. Characterizations of correspondent biomolecule binding properties were simulated and demonstrated. The surface was modified using 3-ammineopropyl-triethoxysilane (3-APTES) and biotin-N-hydroxysuccinimide ester (NHS-biotin). Different concentrations (4.17pM to 41.7nM) of dye-conjugated streptavidin were simultaneously infused through the second microfluidic channels, which lie 90° from the first microfluidic channels. The florescent intensity at the crossover areas showed good agreement with simulations, with sharp ZnO NWs exhibiting the largest dynamic range and the highest fluorescent intensity. We further characterize correspondent protein detection using sharp ZnO NWs. The surfaces of these ZnO NWs were modified with mouse immunoglobulin G (IgG), infused through the second microfluidic channels with dye-conjugated (Alexa 546) anti-mouse IgG in different concentrations. Concentrations ranging from 417fM to 41.7nM can be resolved using sharp ZnO NWs. Finally, multiple protein detection was demonstrated using a five-by-eight m...Continue Reading

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Citations

May 22, 2018·Physical Chemistry Chemical Physics : PCCP·Tiesheng WangFang Xie
Sep 22, 2019·Materials·Muhammad Luqman Mohd NapiMohd Khairul Ahmad
Feb 15, 2016·Micromachines·Jie-Long HeShih-Kang Fan
Dec 2, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Valerii MyndrulIgor Iatsunskyi
Apr 14, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Narsingh R Nirala, Giorgi Shtenberg

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