Fluorescence Excitation Spectroscopy for Phytoplankton Species Classification Using an All-Pairs Method: Characterization of a System with Unexpectedly Low Rank

Applied Spectroscopy
Cameron M RekullyMichael L Myrick

Abstract

An all-pairs method is used to analyze phytoplankton fluorescence excitation spectra. An initial set of nine phytoplankton species is analyzed in pairwise fashion to select two optical filter sets, and then the two filter sets are used to explore variations among a total of 31 species in a single-cell fluorescence imaging photometer. Results are presented in terms of pair analyses; we report that 411 of the 465 possible pairings of the larger group of 31 species can be distinguished using the initial nine-species-based selection of optical filters. A bootstrap analysis based on the larger data set shows that the distribution of possible pair separation results based on a randomly selected nine-species initial calibration set is strongly peaked in the 410-415 pair separation range, consistent with our experimental result. Further, the result for filter selection using all 31 species is also 411 pair separations; The set of phytoplankton fluorescence excitation spectra is intuitively high in rank due to the number and variety of pigments that contribute to the spectrum. However, the results in this report are consistent with an effective rank as determined by a variety of heuristic and statistical methods in the range of 2-3. The...Continue Reading

References

May 17, 2008·Biometrics·Peter BacchettiMark R Segal
Feb 2, 2010·The Review of Scientific Instruments·Laura S HillMichael L Myrick
May 4, 2011·Angewandte Chemie·Krzysztof Kazimierczuk, Vladislav Yu Orekhov
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Jan 1, 1998·Analytical Chemistry·M P NelsonM L Myrick
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Methods Mentioned

BETA
fluorescence excitation spectroscopy
flow cytometry
fluorescence
fluorescence imaging
PCA
light microscopy

Software Mentioned

FlowCytobot

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