PMID: 9641314Jun 26, 1998Paper

Fluorescence imaging of Na+ influx via P2X receptors in cochlear hair cells

Hearing Research
G D HousleyP R Thorne

Abstract

The adenosine 5'-triphosphate (ATP)-activated membrane conductance, mediated by P2X receptors, was examined in isolated guinea-pig cochlear inner and outer hair cells. Photo-activated release of caged-ATP elicted a 30-ms latency inwardly rectifying non-selective cation conductance, blocked by the P2X receptor antagonist pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid (PPADS; 10-100 microM), consistent with the direct activation of ATP-gated ion channels. A K(Ca) conductance in the inner hair cells (IHC), activated by the entry of Ca2+ through the ATP-gated ion channels, was blocked by including 10 mM 1,2-his(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA) in the internal solution. Real-time confocal slit-scanning fluorescence imaging of Na+ influx through the ATP-gated ion channels was performed using the dye Sodium Green with simultaneous whole-cell recording of membrane currents. The Na+ entry was localized to the endolymphatic surface, with the increase in [Na+]i detected within approximately 200 ms of the onset of the inward current response. Within 600 ms Na+ had diffused throughout the cell cytoplasm with the exception of the subnuclear region of the outer hair cells. Correlation of voltage-clamp measuremen...Continue Reading

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Citations

Sep 2, 2010·Purinergic Signalling·Ken Ito, Didier Dulon
Sep 2, 2010·Purinergic Signalling·Yan Zhu, Hong-Bo Zhao
Sep 2, 2010·Purinergic Signalling·Lin-Chien HuangGary D Housley
Jun 28, 2000·Journal of the Autonomic Nervous System·G D Housley, P R Thorne
Sep 5, 2002·Hearing Research·Srdjan M VlajkovicGary D Housley
Sep 19, 2003·Hearing Research·T SuetaD Robertson
Oct 8, 1999·Hearing Research·G M WatsonJ J Repass
Jan 11, 2008·The Journal of Laryngology and Otology·R Ramírez-CamachoJ Nevado
Jul 20, 2000·Clinical and Experimental Pharmacology & Physiology·G D Housley
Apr 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Gary D HousleyAllen F Ryan
Oct 18, 2008·Science·Pau Gorostiza, Ehud Y Isacoff
Jun 9, 2000·Annual Review of Neuroscience·R A Eatock
Feb 22, 2014·BMC Physiology·Calvin WuErnest J Moore
Mar 11, 2009·Neuroscience·M A CrumlingR K Duncan
Mar 21, 2003·Biochemical and Biophysical Research Communications·Takeshi KubotaKotaro Oka
May 31, 2011·Hearing Research·Sung Huhn Kim, Daniel C Marcus
Nov 6, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Srdjan M VlajkovicGary D Housley
Jun 15, 2006·The Journal of Membrane Biology·G D HousleyE N Yamoah
Feb 4, 2005·American Journal of Physiology. Cell Physiology·Xiaorui ShiAlfred L Nuttall
Sep 21, 2007·Molecular BioSystems·Pau Gorostiza, Ehud Isacoff
Aug 12, 2003·Bulletin of Entomological Research·L V LainéD J Wright
Sep 17, 2008·Annual Review of Entomology·Edward L Vargo, Claudia Husseneder
Apr 5, 2001·American Journal of Physiology. Cell Physiology·E M HurK T Kim
Jan 10, 2021·International Journal of Molecular Sciences·Fernando Aleixandre-CarreraAlexander M Binshtok

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