Fluorescence immunosensor based on p-acid-encapsulated silica nanoparticles for tumor marker detection

The Analyst
Mei YanXianrang Song

Abstract

A novel, simple and highly sensitive amplified fluorescence label-free immunosensor by using p-acid-encapsulated silica nanomaterials has been developed for the first time. 4,4'-(2,5-Dimethoxy-1,4-phenylene)bis(ethyne-2,1-diyl)dibenzoic acid (p-acid) and p-acid-encapsulated silica were prepared, and characterized by Fourier transform infrared spectroscopy, nuclear magnetic resonance, ultraviolet visible spectroscopy (UV-vis) and fluorescence spectroscopy. In layer-by-layer self-assembling processes using (3-aminopropyl)triethoxysilane, p-acid@SiO(2) was assembled on the glass substrate. Antibody was immobilized on the surface of p-acid@SiO(2) with N,N'-carbonyldiimidazole. The functional nanomaterials present good analytical properties with a calibration range of 0.1-100 ng mL(-1), and allow the detection of carcinoembryonic antigen (CEA) at a concentration as low as 0.04 ng mL(-1). What is important is that the as-synthesized p-acid@SiO(2) nanomaterials could be further extended for the detection of other biomarkers or biocompounds.

References

Feb 18, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Jiehua LinHuangxian Ju
Mar 15, 2005·Nature Biotechnology·Monya Baker
Sep 7, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·P YangN Murase
Oct 29, 2010·Journal of the American Chemical Society·Kazuki KiyoseTetsuo Nagano
Jan 26, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Séverine LechevallierJeannette Dexpert-Ghys

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