Fluorescence in situ hybridisation (FISH) accelerates identification of Gram-positive cocci in positive blood cultures

International Journal of Antimicrobial Agents
Dorothee Maria GescherAnnette Moter

Abstract

Sepsis is a life-threatening disease with a high mortality rate. Rapid identification of blood culture isolates plays a crucial role in adequate antimicrobial therapy in sepsis patients. To accelerate microbiological diagnosis, a comprehensive panel of oligonucleotide probes for fluorescence in situ hybridisation (FISH) targeting Gram-positive cocci was compiled and evaluated on 428 positive blood culture specimens. By combining genus-specific and species-specific probes, the assay allowed discrimination of staphylococci, streptococci and enterococci as well as differentiation of therapy-relevant pathogens such as Staphylococcus aureus and Enterococcus faecium/durans. Furthermore, the newly designed FISH probes STREP2, ENCO and GRANU targeted Streptococcus pneumoniae/mitis, Enterococcus spp. (except E. faecalis) and Granulicatella adiacens group, respectively. The FISH assay achieved an overall sensitivity of 98.65% and a specificity of 99.0% and therefore allowed rapid and reliable molecular identification of Gram-positive cocci in blood culture specimens.

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Citations

Feb 11, 2011·Current Infectious Disease Reports·Annette MoterDinah Schmiedel
Jul 9, 2013·Molecular Diagnosis & Therapy·Stéphane EmonetBegoña Martinez de Tejada
May 23, 2014·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A J M LoonenA J C van den Brule
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Jan 3, 2014·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·D SchmiedelA Moter
Jan 15, 2015·Diagnostic Microbiology and Infectious Disease·Alice TzengKhaled J Saleh
Apr 4, 2012·FEMS Immunology and Medical Microbiology·Luanne Hall-StoodleyThomas Bjarnsholt
Jun 24, 2010·Clinics in Perinatology·Jeanne A Jordan
Jan 29, 2017·Critical Reviews in Microbiology·Hagen FrickmannSven Poppert
Apr 6, 2011·Expert Review of Molecular Diagnostics·Bernard La Scola
Sep 8, 2010·Expert Review of Anti-infective Therapy·Mohan VenkateshJames Versalovic
Apr 3, 2010·Molecular Diagnosis & Therapy·Julianna Kurlenda, Mariusz Grinholc
Feb 6, 2019·European Journal of Microbiology & Immunology·Annalena ReitzHagen Frickmann
Oct 24, 2018·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Annette MoterThorsten Wiech
Feb 14, 2021·International Journal of Medical Microbiology : IJMM·Dustin GreveJudith Kikhney
Jan 9, 2021·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Felix SchoenrathAnnette Moter

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