Fluorescence in situ hybridization (FISH) analysis of melanocytic nevi and melanomas: sensitivity, specificity, and lack of association with sentinel node status

International Journal of Surgical Pathology
Yuqiang FangKlaus Busam

Abstract

A 4-color fluorescence in situ hybridization (FISH) assay, using probes to chromosomes 11q, 6p, 6q, and 6 cent, has recently been proposed as an ancillary tool for the diagnosis of melanoma. The authors report herein their experience with this assay. To determine the sensitivity and specificity of the assay for histopathologically unequivocal cases, they analyzed 50 melanocytic nevi, 50 primary melanomas, and 15 metastatic melanomas. Of 50 melanocytic nevi, 47 were FISH negative on initial readout (test sensitivity, 94%); 49 were FISH negative after correction for tetraploidy (test specificity, 98%). Of 50 primary melanomas, 41 were FISH positive (test sensitivity, 82%). Of 15 metastatic lesions, 13 were FISH positive (test sensitivity, 85%). Of the 9 FISH-negative melanomas, 6 metastasized. The tumors of the 5 patients who had survived thick primary melanoma for more than 5 years without recurrence were all FISH positive. Half of the patients whose primary melanoma was tested by FISH had undergone sentinel lymph node (SLN) biopsy. When the authors compared the FISH results of those 25 melanomas with the SLN status, no statistically significant correlation was found. These findings document limitations of the current FISH assay...Continue Reading

References

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Citations

Nov 6, 2012·Journal of Skin Cancer·Nitika DabasJames M Grichnik
Dec 18, 2013·Seminars in Diagnostic Pathology·Klaus J Busam
Oct 2, 2012·Dermatologic Clinics·Pedram Gerami, Klaus J Busam
May 20, 2015·Journal of the American Academy of Dermatology·Jordon MarchDouglas Grossman
Oct 24, 2017·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Courtney J EnsslinAnthony M Rossi
Apr 23, 2020·The American Journal of Surgical Pathology·Cecilia LezcanoKlaus J Busam
Mar 22, 2016·The American Journal of Dermatopathology·Gerardo Ferrara, Anna Chiara De Vanna
Apr 6, 2017·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Jennifer S KoLoren E Clarke

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Methods Mentioned

BETA
biopsy
chromosomal aberrations

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