Abstract
Malaria is a responsible for approximately 600 thousand deaths worldwide every year. Appropriate and timely treatment of malaria can prevent deaths but is dependent on accurate and rapid diagnosis of the infection. Currently, microscopic examination of the Giemsa stained blood smears is the method of choice for diagnosing malaria. Although it has limited sensitivity and specificity in field conditions, it still remains the gold standard for the diagnosis of malaria. Here, we report the development of a fluorescence in situ hybridization (FISH) based method for detecting malaria infection in blood smears and describe the use of an LED light source that makes the method suitable for use in resource-limited malaria endemic countries. The Plasmodium Genus (P-Genus) FISH assay has a Plasmodium genus specific probe that detects all five species of Plasmodium known to cause the disease in humans. The P. falciparum (PF) FISH assay and P. vivax (PV) FISH assay detect and differentiate between P. falciparum and P. vivax respectively from other Plasmodium species. The FISH assays are more sensitive than Giemsa. The sensitivities of P-Genus, PF and PV FISH assays were found to be 98.2%, 94.5% and 98.3%, respectively compared to 89.9%, 83.3...Continue Reading
References
Mar 10, 1989·Science·E F DeLongN R Pace
Jan 1, 1987·Journal of Molecular Evolution·E GilsonM Hofnung
Jan 1, 1996·Clinical and Diagnostic Laboratory Immunology·J S ShahD J Lane
Aug 17, 1999·Journal of Clinical Microbiology·H StenderS E Godtfredsen
Mar 7, 2001·Journal of Clinical Microbiology·P HongmaneeO F Rasmussen
Mar 30, 2002·Journal of Clinical Microbiology·Laila F NimriCharles B Beard
May 31, 2002·Journal of Clinical Microbiology·Susan RigbyHenrik Stender
Jun 9, 2004·Journal of Clinical Microbiology·Momar NdaoBrian J Ward
Sep 7, 2004·Expert Review of Molecular Diagnostics·Mohamed J E M F Mabruk
Apr 9, 2005·Journal of Clinical Microbiology·Allison L St AmandNorman R Pace
Oct 7, 2005·Journal of Clinical Microbiology·Allison L St AmandNorman R Pace
Nov 2, 2005·Parasitology Research·Vanessa LemosOlga Matos
Mar 7, 2006·Journal of Clinical Microbiology·Stephanie P JohnstonAlexandre J da Silva
Oct 6, 2006·Journal of Clinical Microbiology·Michael LefmannAnnette Moter
Jun 3, 2009·The Korean Journal of Parasitology·Noppadon TangpukdeeSrivicha Krudsood
Jul 21, 2010·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Anders Björkman, Andreas Mårtensson
Apr 20, 2012·PloS One·Frank BaidenDaniel Chandramohan
Jul 5, 2012·American Journal of Health-system Pharmacy : AJHP : Official Journal of the American Society of Health-System Pharmacists·W Klugh Kennedy, Allyson M Berg
Apr 17, 2013·BMC Microbiology·António MachadoNuno Cerca
Jun 16, 2014·Malaria Journal·Fanny JoannyBenjamin Mordmüller
Jul 10, 2014·BMC Infectious Diseases·Bonnie MeatherallDylan R Pillai
Jul 25, 2014·Journal of Clinical Microbiology·Jong-Han LeeChae Seung Lim
Sep 4, 2014·Frontiers in Public Health·Ranjan Ramasamy
Mar 5, 2016·MMWR. Surveillance Summaries : Morbidity and Mortality Weekly Report. Surveillance Summaries·Karen A CullenUNKNOWN Centers for Disease Control and Prevention (CDC)
Citations
Sep 23, 2016·Frontiers in Cell and Developmental Biology·Chenghua CuiPeining Li
Mar 28, 2017·Vector Borne and Zoonotic Diseases·Josipa KulešMangesh Bhide
Apr 12, 2017·PloS One·Jyotsna ShahRanjan Ramasamy
Jun 11, 2020·Diagnostics·Jyotsna S ShahRanjan Ramasamy
Aug 26, 2020·Veterinary Pathology·Kirsten HülskötterPeter Wohlsein
Jun 20, 2018·Malaria Journal·Muneaki HashimotoMasatoshi Kataoka
Jul 26, 2017·Malaria Journal·Joseph OsogaElizabeth Wanja
Jul 21, 2017·Parasites & Vectors·Jyotsna ShahRanjan Ramasamy
Jan 10, 2018·BMC Infectious Diseases·Regina KandieKariuki Njaanake
Dec 2, 2017·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Anastasia MavropoulosOlga Ornatsky
Jun 14, 2020·Cells·Weikang Nicholas LinLih Feng Cheow
May 26, 2021·Parasites & Vectors·Xi ChenZhaoqing Yang