Fluorescence in situ hybridization to assess aneuploidy for chromosomes 7 and 8 in hematologic disorders

Cancer Genetics and Cytogenetics
H E WyandtC O'Hara

Abstract

Stored, fixed cell suspensions of bone marrows from 70 patients karyotyped over a three-year period for myelodysplastic syndrome (MDS) or related hematologic conditions were retrospectively studied in two series using centromeric probes for chromosomes 7 and 8. Series I consisted of patient samples with numerical and/or structural abnormalities of chromosomes 7 or 8, matched with chromosomally normal samples from about the same time period. Series II consisted of consecutive MDS patient samples as well as patient samples in which one or more cells had numerical or structural abnormalities of 7 and 8. In both series, probes for chromosomes 7 and 8 were applied in each case and at least 100 nuclei were scored for each probe for the distribution of one, two, or three signals. Twenty-seven cases had clonal abnormalities by routine cytogenetics (RC): 12 with monosomy 7; one with monosomy 8; five with trisomy 8; nine with clonal abnormalities other than 7 or 8 aneuploidy. Eleven cytogenetically normal cases gave abnormal interphase FISH (IF) results; one was subsequently confirmed by metaphase FISH analysis to have a clonal structural abnormality of chromosome 7; one case with a trisomy 8 clone, in remission by RC, showed 35% of cell...Continue Reading

References

Sep 1, 1992·Cancer Genetics and Cytogenetics·W S StanleyP Dinndorf
Mar 1, 1991·Genes, Chromosomes & Cancer·E P ArnoldusM van der Ploeg
Sep 1, 1991·Cancer Genetics and Cytogenetics·M BalazsF M Waldman
Feb 1, 1995·American Journal of Hematology·K J HarrisonD K Kalousek
Mar 1, 1993·Annals of Hematology·R WillemzeJ E Landegent

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Citations

Sep 13, 2006·International Journal of Radiation Biology·I UdroiuC Tanzarella
Oct 21, 1999·Experimental and Molecular Pathology·H F MarkS Mark
Jul 20, 1999·Experimental and Molecular Pathology·H F MarkJ Lathrop
Aug 10, 1999·Luminescence : the Journal of Biological and Chemical Luminescence·P E Stanley, L J Kricka
Sep 8, 2010·Journal of Pediatric Hematology/oncology·Arzu AkyayNuket Yürür-Kutlay

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