Fluorescence lifetime imaging of activatable target specific molecular probes.

Contrast Media & Molecular Imaging
Raphael AlfordHisataka Kobayashi

Abstract

In vivo optical imaging using fluorescently labeled self-quenched monoclonal antibodies, activated through binding and internalization within target cells, results in excellent target-to-background ratios. We hypothesized that these molecular probes could be utilized to accurately report on cellular internalization with fluorescence lifetime imaging (FLI). Two imaging probes were synthesized, consisting of the antibody trastuzumab (targeting HER2/neu) conjugated to Alexa Fluor750 in ratios of either 1:8 or 1:1. Fluorescence intensity and lifetime of each conjugate were initially determined at endosomal pHs. Since the 1:8 conjugate is self-quenched, the fluorescence lifetime of each probe was also determined after exposure to the known dequencher SDS. In vitro imaging experiments were performed using 3T3/HER2(+) and BALB/3T3 (HER2(-)) cell lines. Changes in fluorescence lifetime correlated with temperature- and time-dependent cellular internalization. In vivo imaging studies in mice with dual flank tumors [3T3/HER2(+) and BALB/3T3 (HER2(-))] detected a minimal difference in FLI. In conclusion, fluorescence lifetime imaging monitors the internalization of target-specific activatable antibody-fluorophore conjugates in vitro. Chall...Continue Reading

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Citations

Jan 18, 2013·Journal of Biomedical Optics·Yuting LinGultekin Gulsen
May 23, 2012·Journal of Biomedical Optics·Craig J GoergenAnand T N Kumar
Jul 1, 2011·Drug Discovery Today. Technologies·Kai Licha, Ute Resch-Genger
Nov 23, 2011·Cancer Letters·Sukhwinder KaurSurinder K Batra
Sep 11, 2010·Contrast Media & Molecular Imaging
May 4, 2011·Molecular Oncology·Siver Andreas MoestueIngrid Susann Gribbestad

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