Fluorescence lifetime imaging of intracellular magnesium content in live cells

The Analyst
Azzurra SargentiStefano Iotti

Abstract

The first detailed analysis of FLIM applications for Mg cell imaging is presented. We employed the Mg-sensitive fluorescent dye named DCHQ5, a derivative of diaza-18-crown-6 ethers appended with two 8-hydroxyquinoline groups, to perform fluorescence lifetime imaging in control and Mg deprived SaOS-2 live cells, which contain different concentrations of magnesium. We found that the lifetime maps are almost uniform all over the cells and, most relevantly, we showed that the ratio of the amplitude terms is related to the magnesium intracellular concentration.

References

Apr 24, 2001·Trends in Cell Biology·F S WoutersP I Bastiaens
Feb 13, 2003·The Journal of Pathology·Paul J TadrousGordon W H Stamp
Jan 5, 2006·Journal of the American Chemical Society·Giovanna FarruggiaFederica I Wolf
Mar 31, 2006·Environmental Science & Technology·Sibylle M Kaiser, Beate I Escher
Apr 4, 2009·Journal of Biophotonics·James McGintyPaul M W French
Nov 19, 2017·Analytical and Bioanalytical Chemistry·Emil MalucelliStefano Iotti

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Methods Mentioned

BETA
fluorescence imaging
confocal microscopy
imaging techniques

Software Mentioned

FLIM

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