Fluorescence Lifetime Imaging Ophthalmoscopy (FLIO) of Macular Pigment

Investigative Ophthalmology & Visual Science
Lydia SauerPaul S Bernstein

Abstract

To describe different patterns of macular pigment (MP) seen in fluorescence lifetime imaging ophthalmoscopy (FLIO) and to analyze ex vivo fluorescence characteristics of carotenoids. A total of 31 eyes of young healthy subjects, 4 eyes from patients with albinism, 36 eyes with macular telangiectasia type 2 (MacTel), 24 eyes with retinitis pigmentosa, and 1 eye with a macular hole were included in this clinic-based, cross-sectional study. All subjects underwent Heidelberg Engineering FLIO and MP measurements (dual-wavelength autofluorescence). Fundus autofluorescence (FAF) lifetimes of a 30° retinal field were detected in two spectral channels (SSC: 498-560 nm; LSC: 560-720 nm), and amplitude-weighted mean fluorescence lifetimes (τm) were calculated. Additionally, autofluorescence lifetimes of known dilutions of lutein and zeaxanthin were measured in a cuvette in free- and protein-associated states. MP shows a significant inverse correlation to foveal FAF lifetimes measured with FLIO (SSC: r = -0.608; P < 0.001). Different distribution patterns can be assigned to specific disease-related changes. Two patients with albinism, who did not have MP, were found to be missing short FAF lifetimes. In solvent, lutein and zeaxanthin show ...Continue Reading

References

Aug 1, 1978·The British Journal of Ophthalmology·Z Gregor
Nov 21, 1997·Archives of Ophthalmology·E EzraZ J Gregor
Jul 16, 2003·Archives of Ophthalmology·Da-You ZhaoPaul S Bernstein
Jul 29, 2003·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Henrike WüstemeyerSebastian Wolf
Nov 18, 2003·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·M TrieschmannD Pauleikhoff
Dec 1, 2004·Journal of Biomedical Optics·Dietrich SchweitzerE R Gaillard
Aug 2, 2005·Applied Spectroscopy·Igor V ErmakovWerner Gellermann
Jul 4, 2006·Journal of AAPOS : the Official Publication of the American Association for Pediatric Ophthalmology and Strabismus·Patti S HarveyC Gail Summers
Sep 21, 2006·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Mohsen SharifzadehWerner Gellermann
Mar 30, 2007·Microscopy Research and Technique·D SchweitzerA Bergmann
May 28, 2010·Investigative Ophthalmology & Visual Science·Verônica Castro LimaJuliana Sallum
Nov 26, 2010·Current Molecular Medicine·J R SparrowC P Hamel
Jan 5, 2011·Journal of Biomedical Optics·Dietrich SchweitzerSebastian Wolf
May 25, 2012·Journal of Microscopy·W Becker
Aug 18, 2012·Investigative Ophthalmology & Visual Science·Simona Degli EspostiAnthony G Robson
Mar 28, 2013·Current Eye Research·Matthias KlemmDietrich Schweitzer
Mar 1, 2014·Investigative Ophthalmology & Visual Science·Catherine Creuzot-GarcherAlain M Bron
Jul 16, 2014·Journal of Alzheimer's Disease : JAD·John M NolanStephen Beatty
Oct 18, 2014·Journal of Optometry·Christopher M Putnam, Pauline J Bland
Nov 20, 2014·Journal of Alzheimer's Disease : JAD·John M NolanStephen Beatty
Mar 15, 2015·Journal of Biomedical Optics·Dietrich SchweitzerJens Dawczynski
May 30, 2015·Investigative Ophthalmology & Visual Science·Chantal DysliMartin S Zinkernagel
Jul 25, 2015·Investigative Ophthalmology & Visual Science·Lydia SauerSven Peters
Oct 18, 2015·Experimental Eye Research·Yulia WolfsonHendrik P N Scholl
Mar 5, 2016·Investigative Ophthalmology & Visual Science·Chantal DysliMartin S Zinkernagel
Apr 20, 2016·The British Journal of Ophthalmology·Kwadwo Owusu AkuffoStephen Beatty
Aug 16, 2016·Acta Ophthalmologica·Johanna SchmidtMartin Hammer
Jan 19, 2017·Retina·Chantal DysliMartin S Zinkernagel
Jul 5, 2017·Progress in Retinal and Eye Research·Chantal DysliMartin S Zinkernagel
Jul 21, 2017·Investigative Ophthalmology & Visual Science·Christopher D ConradyPaul S Bernstein
Jun 28, 2018·Translational Vision Science & Technology·Karl M AndersenPaul S Bernstein
Jul 20, 2018·Investigative Ophthalmology & Visual Science·Lydia SauerPaul S Bernstein

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Citations

Jun 28, 2018·Translational Vision Science & Technology·Karl M AndersenPaul S Bernstein
Jul 20, 2018·Investigative Ophthalmology & Visual Science·Lydia SauerPaul S Bernstein
Sep 6, 2018·Journal of Biomedical Optics·Lydia SauerMartin Hammer
May 13, 2020·Nutrients·Justyna WidomskaWitold K Subczynski
May 16, 2020·Proceedings of the National Academy of Sciences of the United States of America·Binxing LiPaul S Bernstein
Mar 21, 2019·Biomedical Optics Express·Matthias KlemmJens Haueisen
May 16, 2019·Annual Review of Nutrition·Lydia SauerPaul S Bernstein
Oct 17, 2020·Translational Vision Science & Technology·Alexandra S VitalePaul S Bernstein
Aug 8, 2020·Progress in Retinal and Eye Research·Steffen Schmitz-ValckenbergFrank G Holz
May 27, 2021·The Journal of Physical Chemistry. B·Rafal LuchowskiWieslaw I Gruszecki

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Software Mentioned

ImageJ
FLIMX
SPCImage
SPSS
FLIO

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