Fluorescence lifetime snapshots reveal two rapidly reversible mechanisms of photoprotection in live cells of Chlamydomonas reinhardtii.

Proceedings of the National Academy of Sciences of the United States of America
Kapil AmarnathGraham R Fleming

Abstract

Photosynthetic organisms avoid photodamage to photosystem II (PSII) in variable light conditions via a suite of photoprotective mechanisms called nonphotochemical quenching (NPQ), in which excess absorbed light is dissipated harmlessly. To quantify the contributions of different quenching mechanisms to NPQ, we have devised a technique to measure the changes in chlorophyll fluorescence lifetime as photosynthetic organisms adapt to varying light conditions. We applied this technique to measure the fluorescence lifetimes responsible for the predominant, rapidly reversible component of NPQ, qE, in living cells of Chlamydomonas reinhardtii. Application of high light to dark-adapted cells of C. reinhardtii led to an increase in the amplitudes of 65 ps and 305 ps chlorophyll fluorescence lifetime components that was reversed after the high light was turned off. Removal of the pH gradient across the thylakoid membrane linked the changes in the amplitudes of the two components to qE quenching. The rise times of the amplitudes of the two components were significantly different, suggesting that the changes are due to two different qE mechanisms. We tentatively suggest that the changes in the 65 ps component are due to charge-transfer quen...Continue Reading

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Citations

Jun 25, 2013·Photosynthesis Research·Julia ZaksGraham R Fleming
May 30, 2013·Proceedings of the National Academy of Sciences of the United States of America·Ryutaro Tokutsu, Jun Minagawa
Jul 3, 2013·Proceedings of the National Academy of Sciences of the United States of America·Matthew D BrooksKrishna K Niyogi
Mar 13, 2015·Photosynthesis Research·Emily J Sylak-GlassmanGraham R Fleming
Nov 6, 2015·European Biophysics Journal : EBJ·Arne S KristoffersenSvein R Erga
Mar 12, 2015·The Plant Journal : for Cell and Molecular Biology·Erika EricksonKrishna K Niyogi
Apr 16, 2013·Current Opinion in Plant Biology·Krishna K Niyogi, Thuy B Truong
Nov 26, 2014·Proceedings of the National Academy of Sciences of the United States of America·Emily J Sylak-GlassmanGraham R Fleming
Feb 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Caner ÜnlüHerbert van Amerongen
Jun 24, 2016·Proceedings of the National Academy of Sciences of the United States of America·Emine DincRoberta Croce
Jan 14, 2017·The Journal of Physical Chemistry. B·Takayuki UwadaHiroshi Masuhara
May 3, 2018·Optics Express·Florian RoussetAndrea Farina
Jun 25, 2017·Photosynthesis Research·Fatemeh Azadi-ChegeniAnjali Pandit
May 28, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lloyd Donaldson
Nov 28, 2018·Photosynthesis Research·Volha U ChukhutsinaRoberta Croce
Feb 28, 2019·Proceedings of the National Academy of Sciences of the United States of America·Soomin ParkGraham R Fleming
May 2, 2019·The Journal of Physical Chemistry Letters·Gabriel de la Cruz ValbuenaGiulio Cerullo
Oct 31, 2017·The Journal of Physical Chemistry Letters·Soomin ParkGraham R Fleming

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